Libkindia masarykiana gen. et sp nov., Yurkovia mendeliana gen. et sp nov and Leucosporidium krtinense f.a. sp nov., isolated from temperate forest soils

One hundred and ninety-eight isolates of soil yeasts were isolated from mixed temperate forests in the Czech Republic, and their abundance and distribution in the litter and soil were evaluated using amplicon sequencing of soil fungal communities. Abundant taxa with no close identified hits were selected for further characterization as potential novel species of yeasts. Phylogenetic analyses using sequences of the D1/D2 domain, the ITS region and RPB1 and TEF1 genes support the recognition of the following three novel species belonging to the subphylum Pucciniomycotina, class Microbotryomycetes: Leucosporidium krtinense f.a. sp. nov. (type strain PYCC 6879(T)=KT96(T)=CBS 14304(T)=DSM 101892(T)), Yurkovia mendeliana sp. nov. (type strain PYCC 6884(T)=KT152(T)=CBS 14273(T)=DSM 101889(T)) and Libkindia masarykiana sp. nov. (type strain PYCC 6886(T)=KT310(1)=CBS 14275(1)=DSM 101891(1)). Since the latter two novel taxa cannot be assigned to existing genera, two new genera, Libkindia gen. nov. and Yurkovia gen. nov., are also described.