Novel pulse duplicating bioreactor system for tissue-engineered vascular construct

被引：37

作者：

Narita, Y

Hata, KI

Kagami, H

Usui, A

Ueda, M

Ueda, Y

机构：

[1] Nagoya Univ, Dept Cardiothorac Surg, Grad Sch Med, Showa Ku, Nagoya, Aichi 4668550, Japan

[2] Nagoya Univ, Dept Tissue Engn, Sch Med, Nagoya, Aichi 4668550, Japan

[3] Nagoya Univ Hosp, Ctr Genet & Regenerat Med, Nagoya, Aichi, Japan

[4] Nagoya Univ, Dept Oral & Maxillofacial Surg, Grad Sch Med, Nagoya, Aichi, Japan

来源：

TISSUE ENGINEERING | 2004年 / 10卷 / 7-8期

关键词：

D O I：

10.1089/ten.2004.10.1224

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Cell culture in a biomimetic environment is known to improve the mechanical endurance of tissue-engineered cardiovascular components. Our goal was to generate a bioreactor that can reproduce a wide range of pulsatile flows with a completely physiological pressure profile. The morphology and biochemical properties of tissue-engineered products were also studied to test the usefulness of this novel bioreactor. The combination of an outflow valve, compliance chamber, and resistant clamps together with a balloon pumping system was able to successfully reproduce both physiological systolic and diastolic pressures. The compliance chamber was especially effective in transforming the original peaky pressure waveform into a physiological pressure profile. The tissues, cultured under a physiological pressure waveform with pulsatile flow, presented widely distributed cells in close contact with each other. They also showed significantly higher cell numbers, total protein content, and proteoglycan-glycosaminoglycan content than cultured tissues under a peaky pressure wave or under static conditions. This new bioreactor system is suitable for evaluating a favorable environment for tissue-engineered cardiovascular components.

引用

页码：1224 / 1233

页数：10

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