Inhibition of IL-6 and IL-8 production in LPS-stimulated human gingival fibroblasts by glycyrrhizin via activating LXRα

被引：9
|
作者：
Zhang, Na [1 ,2 ]
Lv, Hui [2 ]
Shi, Bo-Hong [2 ]
Hou, Xin [2 ]
Xu, Xin [1 ]
机构：
[1] Shandong Univ, Sch Stomatol, Shandong Prov Key Lab Oral Tissue Regenerat, Jinan, Shandong, Peoples R China
[2] Peoples Hosp Rizhao, Dept Stomatol, Rizhao, Shandong, Peoples R China
来源：
MICROBIAL PATHOGENESIS | 2017年 / 110卷
关键词：
Glycyrrhizin; Human gingival fibroblasts; IL-8; NF-kappa B; LXR alpha; LIVER X RECEPTORS; PERIODONTAL-DISEASE; KAPPA-B; INFLAMMATORY RESPONSE; NUCLEAR RECEPTORS; LIPOPOLYSACCHARIDE; EXPRESSION; INJURY; CELLS; MICE;
D O I：
10.1016/j.micpath.2017.06.021
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The aim of this study was to clarify the anti-inflammatory effects and its molecular mechanism of glycyrrhizin on LPS-stimulated human gingival fibroblasts (HGFs), which will be of benefit for periodontitis treatment. An MTT assay was performed to assess the effects of glycyrrhizin on cellular viability. The levels of IL-6 and IL-8 were measured by ELISA. The expression of iNOS, COX-2, NF-kappa B, and LXR alpha were detected by western blot analysis. The results showed that glycyrrhizin significantly inhibited LPS-induced IL-6 and IL-8 production, as well as COX-2 and iNOS expression. LPS-induced NF-kappa B activation in HGFs was also inhibited by treatment of glycyrrhizin. Furthermore, glycyrrhizin increased the expression of LXR alpha in a concentration-dependent manner. In addition, the inhibition of glycyrrhizin on IL-6 and IL-8 production was reversed by LXR alpha inhibitor GGPP. In conclusion, these results indicated that glycyrrhizin exhibited its anti-inflammatory effects in HGFs by activating LXR alpha. (C) 2017 Elsevier Ltd. All rights reserved.
引用
收藏
页码：135 / 139
页数：5
相关论文
共 50 条
  • [41] Effects of prostaglandin E2 on LPS-induced production of IL-8 by human gingival fibroblasts in vitro.
    Fujii, T
    Geinoz, A
    Oido, M
    Kowashi, Y
    Baehni, PC
    JOURNAL OF DENTAL RESEARCH, 1998, 77 : 866 - 866
  • [42] PKC-dependent IL-6 production and inhibition of IL-8 production by PKC activation in normal human skin fibroblasts under extremely high hydrostatic pressure
    Koyama, S
    Aizawa, M
    EXTREMOPHILES, 2002, 6 (05) : 413 - 418
  • [43] Sustained inhibition of IL-6 and IL-8 expression by decoy ODN to NF-κB delivered through respirable large porous particles in LPS-stimulated cystic fibrosis bronchial cells
    De Stefano, Daniela
    Ungaro, Francesca
    Giovino, Concetta
    Polimeno, Antonio
    Quaglia, Fabiana
    Carnuccio, Rosa
    JOURNAL OF GENE MEDICINE, 2011, 13 (04): : 200 - 208
  • [44] PKC-dependent IL-6 production and inhibition of IL-8 production by PKC activation in normal human skin fibroblasts under extremely high hydrostatic pressure
    Sumihiro Koyama
    Masuo Aizawa
    Extremophiles, 2002, 6 : 413 - 418
  • [45] EARLY DOWN-REGULATION OF TNF PRODUCTION BY LPS TOLERANCE IN HUMAN MONOCYTES - COMPARISON WITH IL-1-BETA, IL-6, AND IL-8
    MENGOZZI, M
    FANTUZZI, G
    SIRONI, M
    BIANCHI, M
    FRATELLI, M
    PERI, G
    BERNASCONI, S
    GHEZZI, P
    LYMPHOKINE AND CYTOKINE RESEARCH, 1993, 12 (04): : 231 - 236
  • [46] Nitazoxanide suppresses IL-6 production in LPS-stimulated mouse macrophages and TG-injected mice
    Hong, Seong Keun
    Kim, Hee Joo
    Song, Chang Seon
    Choi, In Soo
    Lee, Joong Bok
    Park, Seung Yong
    INTERNATIONAL IMMUNOPHARMACOLOGY, 2012, 13 (01) : 23 - 27
  • [47] Rho-kinase inhibitor Y-27632 downregulates LPS-induced IL-6 and IL-8 production via blocking p38 MAPK and NF-κB pathways in human gingival fibroblasts
    Kang, Wenyan
    Shang, Lingling
    Wang, Ting
    Liu, Hongrui
    Ge, Shaohua
    JOURNAL OF PERIODONTOLOGY, 2018, 89 (07) : 883 - 893
  • [48] Dengue virus infects human endothelial cells and induces IL-6 and IL-8 production
    Huang, YH
    Lei, HY
    Liu, HS
    Lin, YS
    Liu, CC
    Yeh, TM
    AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2000, 63 (1-2): : 71 - 75
  • [49] Homocysteine upregulates IL-6 and IL-8 in human endothelial cells
    Hooper, WC
    Phillips, D
    Evatt, B
    Benson, J
    Renshaw, M
    THROMBOSIS AND HAEMOSTASIS, 1997, : O1609 - O1609
  • [50] Thiol regulation of the production of TNF-α, IL-6 and IL-8 by human alveolar macrophages
    Gosset, P
    Wallaert, B
    Tonnel, AB
    Fourneau, C
    EUROPEAN RESPIRATORY JOURNAL, 1999, 14 (01) : 98 - 105
12345