Single-cell information extraction and viability analysis using automated microscopy

被引：0
|
作者：
Chao, Shih-hui [1 ]
Strovas, Tim J. [1 ]
Zhang, Shile [1 ]
Jones-Isaac, Kendan A. [1 ]
Meldrum, Deirdre R. [1 ]
机构：
[1] Univ Washington, Microscale Life Sci Ctr, Seattle, WA 98195 USA
来源：
2006 IEEE INTERNATIONAL CONFERENCE ON AUTOMATION SCIENCE AND ENGINEERING, VOLS 1 AND 2 | 2006年
基金：
美国国家卫生研究院;
关键词：
automated microscopy; single cell analysis; information extraction; cell viability; microfluidics;
D O I：
暂无
中图分类号：
TP [自动化技术、计算机技术];
学科分类号：
0812 ;
摘要：
We present the use of an automated microscope routine for long-term single cell viability analysis. Murine macrophage cells were monitored for more than 10 hours at physiological conditions. The information of each cell was extracted from time-lapse raw fluorescence and bright-field images to study single cell dynamic behaviors. Two methods were applied to analyze single cell viability: the popular method using live/dead fluorescent dye, and a new morphology-based, dye-free method that estimates viability with optical appearance. Both methods yielded similar death event estimation, indicating the new morphology-based method can be an alternative when using live/dead fluorescent dye is difficult or not allowed.
引用
收藏
页码：33 / +
页数：2
相关论文
共 50 条
  • [41] Recent advances of scanning electrochemical microscopy and scanning ion conductance microscopy for single-cell analysis
    Zhang, Junjie
    Zhu, Tong
    Lang, Jinxin
    Fu, Wenxuan
    Li, Fei
    CURRENT OPINION IN ELECTROCHEMISTRY, 2020, 22 : 178 - 185
  • [42] Inference of single-cell network using mutual information for scRNA-seq data analysis
    Chang, Lan-Yun
    Hao, Ting-Yi
    Wang, Wei-Jie
    Lin, Chun-Yu
    BMC BIOINFORMATICS, 2024, 25 (SUPPL 2):
  • [43] Automated and Miniaturized Pico-Liter Metabolite Extraction System for Single-Cell Mass Spectrometry
    Zhao, Peng
    Cheng, Simin
    Feng, Yongxiang
    Liang, Fei
    Zhang, Xinrong
    Ma, Xiaoxiao
    Wang, Wenhui
    IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING, 2023, 70 (02) : 470 - 478
  • [44] Use of a novel microfluidic disk in the analysis of single-cell viability and the application to Jurkat cells
    Department of Bioinformatics, Graduate School of Engineering, Soka University, 1-236 Tangi, Hachioji, Tokyo 192-8577, Japan
    J. Biosci. Bioeng., 1 (98-101):
  • [45] Interactive single-cell data analysis using Cellar
    Euxhen Hasanaj
    Jingtao Wang
    Arjun Sarathi
    Jun Ding
    Ziv Bar-Joseph
    Nature Communications, 13
  • [46] Use of a novel microfluidic disk in the analysis of single-cell viability and the application to Jurkat cells
    Kubo, Izumi
    Furutani, Shunsuke
    Matoba, Kenji
    JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2011, 112 (01) : 98 - 101
  • [47] Interactive single-cell data analysis using Cellar
    Hasanaj, Euxhen
    Wang, Jingtao
    Sarathi, Arjun
    Ding, Jun
    Bar-Joseph, Ziv
    NATURE COMMUNICATIONS, 2022, 13 (01)
  • [48] Single-cell manipulation and analysis using microfluidic devices
    Gregory T. Roman
    Yanli Chen
    Pernilla Viberg
    Anne H. Culbertson
    Christopher T. Culbertson
    Analytical and Bioanalytical Chemistry, 2007, 387 : 9 - 12
  • [49] Single-cell manipulation and analysis using microfluidic devices
    Roman, Gregory T.
    Chen, Yanli
    Viberg, Pernilla
    Culbertson, Anne H.
    Culbertson, Christopher T.
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2007, 387 (01) : 9 - 12
  • [50] Single-Cell Analysis Using Hyperspectral Imaging Modalities
    Mehta, Nishir
    Shaik, Shahensha
    Devireddy, Ram
    Gartia, Manas Ranjan
    JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME, 2018, 140 (02):
12345