A human-food web-animal interface on the prevalence of food-borne pathogens (Clostridia and Enterococcus) in mixed veterinary farms

被引：0

作者：

Prisilla, A. ^{[1
]}

Remin, M. Deena ^{[1
]}

Roja, B. ^{[1
]}

Chellapandi, P. ^{[1
]}

机构：

[1] Bharathidasan Univ, Sch Life Sci, Dept Bioinformat, Mol Syst Engn Lab, Tiruchirappalli 620024, Tamil Nadu, India

来源：

FOOD SCIENCE AND BIOTECHNOLOGY | 2019年 / 28卷 / 05期

关键词：

Avian botulism; Poultry disease; Molecular diagnosis; Phylogeny; Clostridium; Enterococcus; DIFFERENT INTESTINAL BACTERIA; ANTIMICROBIAL RESISTANCE; BOTULINUM; DIVERSITY; CATTLE; OUTBREAKS; POULTRY; INFECTION; DIAGNOSIS; BROILER;

D O I：

10.1007/s10068-019-00595-8

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

In the present work, we addressed the impact of a human-food web-animal interface on the prevalence of food-borne pathogens in mixed farms of Tamil Nadu, India. We have isolated and identified six strains of Clostridium sp. and five strains of Enterococcus sp. from food and animal sources disposed near to the veterinary and poultry farms. Phylogenetic relationships of these strains were inferred from their homologies in 16S rDNA sequences and rRNA secondary structures. The strain PCP07 was taxonomically equivalent to C. botulinum confirmed by neurotoxin-specific PCR primers, followed by mouse bioassay. Other Clostridial and Enterococcal isolates have shown a phylogenetic similarity to the C. bifermentans and E. durans isolated from veterinary farms, respectively. Results of our study revealed that a human-food web-animal interface has influenced the disease incidence and prevalence of these isolates in the poultry to veterinary farms, where human food acted as a likely transmittance vehicle for their infections.

引用

页码：1583 / 1591

页数：9