Combined Transplantation of Neural Stem Cells and Olfactory Ensheathing Cells Improves the Motor Function of Rats with Intracerebral Hemorrhage

被引:10
|
作者
Tang, Zhou-Ping [1 ]
Xie, Xue-Wei [1 ]
Shi, Yuan-Hong [1 ]
Liu, Na [1 ]
Zhu, Sui-Qiang [1 ]
Li, Zai-Wang [1 ]
Chen, Yun [1 ]
机构
[1] Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Dept Neurol, Wuhan 430030, Hubei, Peoples R China
基金
中国国家自然科学基金;
关键词
Stem cell; Olfactory bulb; Cell transplantation; Intracerebral hemorrhage; Motor function;
D O I
10.1016/S0895-3988(10)60033-5
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Objective To investigate the effects of combined transplantation of neural stem cells (NSC) and olfactory ensheathing cells (OEC) on the motor function of rats with intracerebral hemorrhage :Methods In three days after a rat model of caudate nucleus hemorrhage was established, NSCs and OEC, NSC, OEC (from embryos of Wistar rats) or normal saline were injected into hematomas of rats in combined transplantation group, NSC group, OEC group, and control group, respectively Damage of neural function was scored before and in 3, 7, 14, 30 days after operation Tissue after transplantation was observed by immunocytochemistry staining Results The scores for the NSC, OEC: and co-transplantation groups were significantly lower in 14 and 30 days after operation than in 3 days after operation (P<0 05) The scores for the NSC and OEC groups were significantly lower than those for the control group only in 30 days after operation (P<0 05), while the difference for the NSC-OEC group was significant in 14 days after operation (P<0 05) Immunocytochemistry staining revealed that the transplanted OEC and NSC could survive, migrate and differentiate into neurons, astrocytes, and oligodendrocytes The number of neural precursor cells was greater in the NSC; and combined transplantation groups than in the control group The number of neurons differentiated from NSC was significantly greater in the co-transplantation group than in the NSC group Conclusion Co-transplantation of NSC and OEC can promote the repair of injured tissue and improve the motor function of rats with intracerebral hemorrhage
引用
收藏
页码:62 / 67
页数:6
相关论文
共 50 条
  • [31] TRANSPLANTATION OF OLFACTORY ENSHEATHING CELLS FOR PERIPHERAL NERVE REGENERATION
    Radtke, Christine
    Kocsis, Jeffery D.
    Vogt, Peter M.
    ESSAYS ON PERIPHERAL NERVE REPAIR AND REGENERATION, 2009, 87 : 405 - 415
  • [32] Repair of corticospinal axons by transplantation of olfactory ensheathing cells
    Raisman, G
    Field, PM
    Li, Y
    EUROPEAN JOURNAL OF NEUROSCIENCE, 2000, 12 : 318 - 318
  • [33] Effect of Tongfu Xingshen capsule on the endogenous neural stem cells of experimental rats with intracerebral hemorrhage
    Cui, Zhizhong
    Liu, Shanshan
    Hou, Lingbo
    Sun, Yifan
    Chen, Haoxuan
    Mao, Hui
    Zhao, Yuanqi
    Qiao, Lijun
    MOLECULAR MEDICINE REPORTS, 2021, 24 (03)
  • [34] Tracking of neural stem cells in rats with intracerebral hemorrhage by the use of 3T MRI
    Chang, Nam Kyu
    Jeong, Yong Yeon
    Park, Jong-Seong
    Jeong, Han-Seong
    Jang, Sujeong
    Jang, Myung Joo
    Lee, Jae Hyuk
    Shin, Sang Soo
    Yoon, Woong
    Chung, Tae Woong
    Kang, Heoung Keun
    KOREAN JOURNAL OF RADIOLOGY, 2008, 9 (03) : 196 - 204
  • [35] Repair of corticospinal axons by transplantation of olfactory ensheathing cells
    Raisman, G
    NEURAL TRANSPLANTATION IN NEURODEGENERATIVE DISEASE: CURRENT STATUS AND NEW DIRECTIONS, 2000, 231 : 94 - 97
  • [36] Combining Adult Stem Cells and Olfactory Ensheathing Cells: The Secretome Effect
    Silva, Nuno A.
    Gimble, Jeffrey M.
    Sousa, Nuno
    Reis, Rui L.
    Salgado, Antonio J.
    STEM CELLS AND DEVELOPMENT, 2013, 22 (08) : 1232 - 1240
  • [37] Effect of olfactory ensheathing cells on differentiation of bone marrow stem cells
    Kim, Su Mi
    Kim, Soon Hee
    Park, Sang Wook
    Oh, A. Young
    Kim, Geun Ah
    Rhee, John M.
    Khang, Gilson
    TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 2008, 5 (01) : 117 - 123
  • [38] DEVELOPMENT OF OLFACTORY ENSHEATHING CELLS FROM THE NEURAL CREST
    Baker, C.
    GLIA, 2011, 59 : S12 - S12
  • [39] Influences of olfactory ensheathing cells transplantation on axonal regeneration in spinal cord of adult rats
    沈慧勇
    唐勇
    吴燕峰
    陈燕涛
    程志安
    ChineseJournalofTraumatology, 2002, (03)
  • [40] Transplantation of olfactory ensheathing cells promotes partial recovery in rats with experimental autoimmune encephalomyelitis
    Li, Jia
    Chen, Weian
    Li, Yu'an
    Chen, Ying
    Ding, Zhangna
    Yang, Dehao
    Zhang, Xu
    INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY, 2015, 8 (09): : 11149 - 11156