People are fascinated by trees since ancient times, by their grandiosity, age and aesthetic beauty. The age of trees is one of the most discussed variables. It is for two reasons. The first reason is the issue of determination of the age of trees itself. The second one is, how old they can be. In general, a method with universal application does not exist. Exact age of only a few trees is known from historical documents. Thus, to determine the age of the tree some of the analytical methods which is based on a measurable attribute must be used. The aim of this work is to determine the age of small-leaved lime (Tilia cordata), which grows in the village Maskovice in the Czech Republic. Opinions on the age of this tree varies widely, ranging between 350 and 900 years. Different non-invasive methods and measurements of the circumference of a tree trunk reported in literature were used for age determination of the tree.