A modification for the calculation of water depth profiles in oil-treated skin by in vivo confocal Raman microscopy

被引：20
|
作者：
Choe, Chunsik [1 ]
Schleusener, Johannes [2 ]
Choe, Sehyok [1 ]
Lademann, Juergen [2 ]
Darvin, Maxim E. [2 ]
机构：
[1] Kim Il Sung Univ, Fac Mat Sci, Biomed Mat Div, Pyongyang, North Korea
[2] Charite Univ Med Berlin, Dept Dermatol Venerol & Allergol, Ctr Expt & Appl Cutaneous Physiol, Charitepl 1, D-10117 Berlin, Germany
来源：
JOURNAL OF BIOPHOTONICS | 2020年 / 13卷 / 01期
关键词：
cosmetics; skin barrier function; skin hydration; stratum corneum; topical application; STRATUM-CORNEUM; PORCINE SKIN; PENETRATION; SPECTROSCOPY; MOISTURIZERS; SPECTRA; LIPIDS;
D O I：
10.1002/jbio.201960106
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
In this study, an extended calculation method for the determination of the water profiles in oil-treated skin is proposed, which is based on the calculation of the ratio between the Raman band intensities of water (3350-3550 cm(-1)) and keratin Amide I at 1650 cm(-1). The proposed method is compared with the conventional method based on the ratio of the Raman band intensities of water (3350-3550 cm(-1)) and keratin at 2930 cm(-1). The conventional method creates artifacts in the depth profiles of the water concentration in oil-treated skin, showing a lower amount of water in the upper and intermediate layers of the stratum corneum, which is due to the superposition of oil- and keratin-related Raman bands at 2930 cm(-1). The proposed extended method shows no artifacts and has the potential to determine the water depth profiles after topical application of formulations on the skin.
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收藏
页数：9
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