A modification for the calculation of water depth profiles in oil-treated skin by in vivo confocal Raman microscopy

被引：20

作者：

Choe, Chunsik ^{[1
]}

Schleusener, Johannes ^{[2
]}

Choe, Sehyok ^{[1
]}

Lademann, Juergen ^{[2
]}

Darvin, Maxim E. ^{[2
]}

机构：

[1] Kim Il Sung Univ, Fac Mat Sci, Biomed Mat Div, Pyongyang, North Korea

[2] Charite Univ Med Berlin, Dept Dermatol Venerol & Allergol, Ctr Expt & Appl Cutaneous Physiol, Charitepl 1, D-10117 Berlin, Germany

来源：

JOURNAL OF BIOPHOTONICS | 2020年 / 13卷 / 01期

关键词：

cosmetics; skin barrier function; skin hydration; stratum corneum; topical application; STRATUM-CORNEUM; PORCINE SKIN; PENETRATION; SPECTROSCOPY; MOISTURIZERS; SPECTRA; LIPIDS;

D O I：

10.1002/jbio.201960106

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, an extended calculation method for the determination of the water profiles in oil-treated skin is proposed, which is based on the calculation of the ratio between the Raman band intensities of water (3350-3550 cm(-1)) and keratin Amide I at 1650 cm(-1). The proposed method is compared with the conventional method based on the ratio of the Raman band intensities of water (3350-3550 cm(-1)) and keratin at 2930 cm(-1). The conventional method creates artifacts in the depth profiles of the water concentration in oil-treated skin, showing a lower amount of water in the upper and intermediate layers of the stratum corneum, which is due to the superposition of oil- and keratin-related Raman bands at 2930 cm(-1). The proposed extended method shows no artifacts and has the potential to determine the water depth profiles after topical application of formulations on the skin.

引用

页数：9

共 45 条

[1] Comment on Choe et al "A modification for the calculation of water depth profiles in oil-treated skin by in vivo Raman microscopy"
Puppels, Gerwin
Caspers, Peter
Nico, Claudio
JOURNAL OF BIOPHOTONICS, 2020, 13 (06)
[2] Response to comment by Puppels et al. on "A modification for the calculation of water depth profiles in oil-treated skin by in vivo Raman microscopy"
Darvin, Maxim E.
Choe, Chunsik
Schleusener, Johannes
Choe, Sehyok
Lademann, Juergen
JOURNAL OF BIOPHOTONICS, 2020, 13 (06)
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Caspers, PJ
Lucassen, GW
Bruining, HA
Puppels, GJ
JOURNAL OF RAMAN SPECTROSCOPY, 2000, 31 (8-9) : 813 - 818
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Caspers, PJ
Lucassen, GW
Puppels, GJ
BIOPHYSICAL JOURNAL, 2003, 85 (01) : 572 - 580
[5] Water Exclusion Layers Probed by Depth Scan Confocal Raman Microscopy
Teschke, Omar
Bonugli, Luiz O.
dos Santos, Marcus V. P.
MICROSCOPY AND MICROANALYSIS, 2013, 19 (05) : 1317 - 1322
[6] Confocal Raman microscopy: how to correct depth profiles considering diffraction and refraction effects
Gallardo, Alberto
Spells, Stephen
Navarro, Rodrigo
Reinecke, Helmut
JOURNAL OF RAMAN SPECTROSCOPY, 2007, 38 (07) : 880 - 884
[7] In vivo confocal Raman microspectroscopy of the skin:: Noninvasive determination of molecular concentration profiles
Caspers, PJ
Lucassen, GW
Carter, EA
Bruining, HA
Puppels, GJ
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2001, 116 (03) : 434 - 442
[8] Confocal Raman microscopy for investigating the penetration of various oils into the human skin in vivo
Choe, ChunSik
Lademann, Juergen
Darvin, Maxim E.
JOURNAL OF DERMATOLOGICAL SCIENCE, 2015, 79 (02) : 176 - 178
[9] Age related depth profiles of human Stratum Corneum barrier-related molecular parameters by confocal Raman microscopy in vivo
Choe, ChunSik
Schleusener, Johannes
Lademann, Juergen
Darvin, Maxim E.
MECHANISMS OF AGEING AND DEVELOPMENT, 2018, 172 : 6 - 12
[10] How Confocal Is Confocal Raman Microspectroscopy on the Skin? Impact of Microscope Configuration and Sample Preparation on Penetration Depth Profiles
Lunter, Dominique Jasmin
SKIN PHARMACOLOGY AND PHYSIOLOGY, 2016, 29 (02) : 92 - 101

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