Characterization of Amyloid β Fibrils with An Aqueous Two-Phase System: Implications of Fibril Formation

被引：7

作者：

Shimanouchi, Toshinori ^{[1
]}

Shimauchi, Naoya ^{[1
]}

Nishiyama, Keiichi ^{[1
]}

Vu, Huong Thi ^{[1
]}

Yagi, Hisashi ^{[2
]}

Goto, Yuji ^{[2
]}

Umakoshi, Hiroshi ^{[1
]}

Kuboi, Ryoichi ^{[1
]}

机构：

[1] Osaka Univ, Grad Sch Engn Sci, Div Chem Engn, Osaka 5608531, Japan

[2] Osaka Univ, Inst Prot Res, Suita, Osaka 5650871, Japan

来源：

SOLVENT EXTRACTION RESEARCH AND DEVELOPMENT-JAPAN | 2010年 / 17卷

基金：

日本学术振兴会;

关键词：

ASSOCIATION; MECHANISM;

D O I：

10.15261/serdj.17.121

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The pathological process of Alzheimer's disease is closely related to amyloid fibril formation by the causative protein, amyloid beta (A beta). The growth behavior of A beta fibrils is predominated by the seeds-monomeric A beta interaction. In this study, the local hydrophobicity of seeds of A beta fibrils was investigated by the aqueous two-phase partitioning method to evaluate the hydrophobic interaction between the seed-monomeric A beta. The seeds showed a high local hydrophobicity relative to the monomer and fibrils. From the fibril growth experiment and the additive effect of Triton X-100, we could demonstrate the contribution of the hydrophobic seeds-monomer interaction to fibril formation.

引用

页码：121 / 128

页数：8

共 50 条

[41] Green speciation of iron using aqueous two-phase system
Campos, Raquel A.
Patricio, Pamela R.
Vargas, Silvia Juliana R.
Da Silva, Luis Henrique M.
Hespanhol, Maria C.
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS, 2018, 90 (02): : 1929 - 1944
[42] Cold cataracts: a naturally occurring aqueous two-phase system
Petitt, P
Forciniti, D
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2000, 743 (1-2): : 431 - 441
[43] Study on Nattokinase-Extraction with two-phase aqueous system
Yang, Yingge
PROCEEDINGS OF THE 5TH INTERNATIONAL CONFERENCE ON INFORMATION ENGINEERING FOR MECHANICS AND MATERIALS, 2015, 21 : 246 - 250
[44] Bioseparation of phycocyanin fromPhormidium tergestinumusing an aqueous two-phase system
Tan, Joo Shun
Abbasiliasi, Sahar
Lalung, Japareng
Tam, Yew Joon
Murugan, Paramasivam
Lee, Chee Keong
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, 2021, 51 (03): : 260 - 266
[45] Photoresponse of an aqueous two-phase system composed of photochromic dextran
Edahiro, Jun-ichi
Sumaru, Kimio
Takagi, Toshiyuki
Shinbo, Toshio
Kanamori, Toshiyuki
LANGMUIR, 2006, 22 (12) : 5224 - 5226
[46] Production of cyclodextrin homologues using aqueous two-phase system
Chang W.-J.
Koo Y.-M.
Park S.-S.
Biotechnology and Bioprocess Engineering, 1997, 2 (2) : 97 - 100
[47] Purification and In Situ Immobilization of Papain with Aqueous Two-Phase System
Li, Mingliang
Su, Erzheng
You, Pengyong
Gong, Xiangyu
Sun, Ming
Xu, Diansheng
Wei, Dongzhi
PLOS ONE, 2010, 5 (12):
[48] Partition of proteins in the aqueous guar/dextran two-phase system
Simonet, F
Garnier, C
Doublier, JL
FOOD HYDROCOLLOIDS, 2000, 14 (06) : 591 - 600
[49] Integration of ultrafiltration into an aqueous two-phase system in the keratinase purification
Sala, Luisa
Gauterio, Gabrielle Victoria
Younan, Felipe Fares
Brandelli, Adriano
Moraes, Caroline Costa
Kalil, Susana Juliano
PROCESS BIOCHEMISTRY, 2014, 49 (11) : 2016 - 2024
[50] Cell separation by an aqueous two-phase system in a microfluidic device
Tsukamoto, Masatoshi
Taira, Shu
Yamamura, Shohei
Morita, Yasutaka
Nagatani, Naoki
Takamura, Yuzuru
Tamiya, Eiichi
ANALYST, 2009, 134 (10) : 1994 - 1998

← 1 2 3 4 5 →