Activity-based fluorescent probes that target phosphatases

被引：50
|
作者：
Zhu, Q
Huang, X
Chen, GYJ
Yao, SQ
机构：
[1] Natl Univ Singapore, Dept Chem, Singapore 117543, Singapore
[2] Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore
来源：
TETRAHEDRON LETTERS | 2003年 / 44卷 / 13期
关键词：
D O I：
10.1016/S0040-4039(03)00348-4
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
We have successfully designed and synthesized two fluorescently-labeled, activity-based probes, Probe I and Probe 2, which were shown to label protein tyrosine phosphatases specifically, as well as other types of phosphatases. The probes were not reactive towards the other non-phosphatase enzymes tested. These probes may find potential applications in large-scale proteomic experiments whereby subclasses of proteins may be selectively identified. (C) 2003 Elsevier Science Ltd. All rights reserved.
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页码：2669 / 2672
页数：4
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