Engineered Exosomes-Mediated Transfer of hsa-miR-320a Overcomes Chemoresistance in Cervical Cancer Cells via Targeting MCL1

被引：6

作者：

Zhou, Jinling ^{[1
]}

Wang, Yuanhe ^{[1
]}

Zhang, Lizhu ^{[2
]}

Chen, Qin ^{[3
]}

Zhu, Xiaojun ^{[1
]}

Jiang, Peiyue ^{[1
]}

Jiang, Nan ^{[4
]}

Zhao, Wei ^{[5
,6
]}

Li, Baohua ^{[1
]}

机构：

[1] Zhejiang Univ, Womens Hosp, Dept Obstet & Gynecol, Sch Med, Hangzhou, Peoples R China

[2] Inst Nanjing Nanxin Pharmaceut Technol Res, Nanjing, Peoples R China

[3] Zhejiang Univ, Womens Hosp, Dept Pathol, Sch Med, Hangzhou, Peoples R China

[4] Zhejiang Univ, Womens Hosp, Dept Clin Lab, Sch Med, Hangzhou, Peoples R China

[5] City Univ Hong Kong, Dept Biomed Sci, Hong Kong, Peoples R China

[6] City Univ Hong Kong, Tung Biomed Sci Ctr, Hong Kong, Peoples R China

来源：

FRONTIERS IN PHARMACOLOGY | 2022年 / 13卷

基金：

中国国家自然科学基金;

关键词：

engineered exosomes; cervical cancer; has-miR-320a; Mcl1; chemoresistance; CISPLATIN-RESISTANCE; MIR-320A;

D O I：

10.3389/fphar.2022.883445

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

In cervical cancer (CC), cisplatin resistance greatly restricts the application in clinical. Here, we report that engineered exosomes-mediated transfer of hsa-miR-320a overcomes chemoresistance in cervical cancer cells via targeting Myeloid Cell Leukemia Sequence 1 (MCL1). In DDP resistant CC tissues, as well as cell lines, it was found that miR-320a expression is lower, engineered miR-320a exosomes were used to attenuate DDP resistance in Hela/DDP and Caski/DDP cells. Mechanistically, we find that MCL1, which is a target of miR-320a, overcomes DDP resistance in Hela/DDP cells and in mice. In conclusion, we report that the engineered miR-320a exosomes is proved to be effective and safe.

引用

页数：10