Real-Time Monitoring of Somatostatin Receptor-cAMP Signaling in Live Pituitary

被引:12
|
作者
Jacobs, Stefan [2 ]
Calebiro, Davide [2 ,3 ]
Nikolaev, Viacheslav O. [2 ,3 ]
Lohse, Martin J. [2 ,3 ]
Schulz, Stefan [1 ]
机构
[1] Univ Jena, Univ Hosp, Inst Pharmacol & Toxicol, D-07747 Jena, Germany
[2] Univ Wurzburg, Inst Pharmacol & Toxicol, D-97078 Wurzburg, Germany
[3] Deutsch Forsch Gemeinschaft Res Ctr Expt Biomed, Rudolf Virchow Ctr, D-97078 Wurzburg, Germany
关键词
CARDIAC MYOCYTES; CYCLIC-AMP; OCTREOTIDE; CELLS; EXPRESSION; INDICATOR; THERAPY;
D O I
10.1210/en.2010-0341
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Fluorescence resonance energy transfer using genetically encoded biosensors has proven to be a powerful technique to monitor the spatiotemporal dynamics of cAMP signals stimulated by G(s)-coupled receptors in living cells. In contrast, real-time imaging of G(i)-mediated cAMP signals under native conditions remains challenging. Here, we describe the use of transgenic mice for cAMP imaging in living pituitary slices and primary pituitary cells. This technique can be widely used to assess the contribution of various pituitary receptors, including individual G(i) protein-coupled somatostatin receptors, to the regulation of cAMP levels under physiologically relevant settings. (Endocrinology 151: 4560-4565, 2010)
引用
收藏
页码:4560 / 4565
页数:6
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