A mechanical cell disruption microfluidic platform based on an on-chip micropump

被引：11

作者：

Cheng, Yinuo ^{[1
]}

Wang, Yue ^{[1
]}

Wang, Zhiyuan ^{[1
]}

Huang, Liang ^{[1
]}

Bi, Mingzhao ^{[1
]}

Xu, Wenxiao ^{[1
]}

Wang, Wenhui ^{[1
]}

Ye, Xiongying ^{[1
]}

机构：

[1] Tsinghua Univ, Dept Precis Instruments, State Key Lab Precis Measurement Technol & Instru, Beijing, Peoples R China

来源：

BIOMICROFLUIDICS | 2017年 / 11卷 / 02期

关键词：

SINGLE-CELL; DNA EXTRACTION; LYSIS; MICROALGAE; ELECTROPORATION; PROTEINS; RECOVERY; RELEASE; DEVICES;

D O I：

10.1063/1.4979100

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cell disruption plays a vital role in detection of intracellular components which contain information about genetic and disease characteristics. In this paper, we demonstrate a novel microfluidic platform based on an on-chip micropump for mechanical cell disruption and sample transport. A 50 mu l cell sample can be effectively lysed through on-chip multi-disruption in 36 s without introducing any chemical agent and suffering from clogging by cellular debris. After 30 cycles of circulating disruption, 80.6% and 90.5% cell disruption rates were achieved for the HEK293 cell sample and human natural killer cell sample, respectively. Profiting from the feature of pump-on-chip, the highly integrated platform enables more convenient and cost-effective cell disruption for the analysis of intracellular components. Published by AIP Publishing.

引用

页数：9

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