Synthesis of stable-isotope (13C and 15N) labeled nucleosides and their applications

NMR studies of stable-isotope labeled oligonucleotides have provided useful information regarding nucleic acid structures, nucleic acid - drug binding, and nucleic acid - protein interactions. For the conformational elucidation of the sugar-phosphate backbone and/or the sugar moieties in nucleic acids, C-13-labeled oligonucleotides are very useful, and for the transformation of nucleic acid - drug binding sight are N-15-labeled oligonucleotides. The present paper has introduced the recent synthetic methods of nucleosides containing either a C-13 labeled sugar moiety or a N-15-labeled exocyclic amine, and applications including our research work.