Activation of cytosolic Slingshot-1 phosphatase by gelsolin-generated soluble actin filaments

被引：5
|
作者：
Takahashi, Katsunori [1 ]
Kanno, Shin-ichiro [2 ]
Mizuno, Kensaku [1 ]
机构：
[1] Tohoku Univ, Grad Sch Life Sci, Dept Biomol Sci, Sendai, Miyagi 9808578, Japan
[2] Tohoku Univ, Inst Dev Aging & Canc, Div Dynam Proteome Canc & Aging, Sendai, Miyagi 9808575, Japan
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2014年 / 454卷 / 03期
关键词：
Slingshot; Gelsolin; Cofilin; Scinderin; Actin oligomers; Phosphatase; COFILIN PHOSPHORYLATION; LIM-KINASE; REORGANIZATION; DEPHOSPHORYLATION; ADF/COFILIN; MECHANISMS; MOTILITY; DYNAMICS; FAMILY; ROLES;
D O I：
10.1016/j.bbrc.2014.10.108
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Slingshot-1 (SSH1) is a protein phosphatase that dephosphorylates and activates cofilin, an actin-severing and -disassembling protein. SSH1 is bound to and activated by F-actin, but not G-actin. SSH1 is accumulated in the F-actin-rich lamellipodium but is also diffusely distributed in the cytoplasm. It remains unknown whether SSH1 is activated by soluble (low-level polymerized) actin filaments in the cytoplasm. In this study, we show that SSH1 binds to gelsolin via actin filaments in the cytosolic fraction. Gelsolin promoted solubilization of actin filaments and SSH1 in cell-free assays and in cultured cells. SSH1 was activated by gelsolin-generated soluble actin filaments. Furthermore, gelsolin enhanced coffin dephosphorylation in neuregulin-stimulated cells. Our results suggest that cytosolic SSH1 forms a complex with gelsolin via soluble actin filaments and is activated by gelsolin-generated soluble actin filaments and that gelsolin promotes stimulus-induced cofilin dephosphorylation through increasing soluble actin filaments, which support SSH1 activation in the cytoplasm. (C) 2014 Elsevier Inc. All rights reserved.
引用
收藏
页码：471 / 477
页数：7
相关论文
共 33 条
  • [21] Hypotonic activation of short ClC3 isoform is modulated by direct interaction between its cytosolic C-terminal tail and subcortical actin filaments
    McCloskey, Diana T.
    Doherty, Lynda
    Dai, Yan-Ping
    Miller, Lisa
    Hume, Joseph R.
    Yamboliev, Ilia A.
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2007, 282 (23) : 16871 - 16877
  • [22] Thrombin activation of human platelets dissociates a complex containing gelsolin and actin from phosphatidylinositide-specific phospholipase C gamma(1)
    Baldassare, JJ
    Henderson, PA
    Tarver, A
    Fisher, GJ
    BIOCHEMICAL JOURNAL, 1997, 324 : 283 - 287
  • [23] Dual Regulation of Cofilin-1 Activity by LIM Kinase and Slingshot-1L Phosphatase Mediates Thrombin-Induced Actin Dynamics and ICAM-1 Expression in Endothelial Cells
    Fazal, F.
    Bijli, K. M.
    Finkelstein, J. N.
    Rahman, A.
    AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2010, 181
  • [24] Fibrillar Amyloid-β1-42 Modifies Actin Organization Affecting the Cofilin Phosphorylation State: A Role for Rac1/cdc42 Effector Proteins and the Slingshot Phosphatase
    Mendoza-Naranjo, Ariadna
    Contreras-Vallejos, Erick
    Henriquez, Daniel R.
    Otth, Carola
    Bamburg, James R.
    Maccioni, Ricardo B.
    Gonzalez-Billault, Christian
    JOURNAL OF ALZHEIMERS DISEASE, 2012, 29 (01) : 63 - 77
  • [25] Activation of Human γδ T Cells by Cytosolic Interactions of BTN3A1 with Soluble Phosphoantigens and the Cytoskeletal Adaptor Periplakin
    Rhodes, David A.
    Chen, Hung-Chang
    Price, Amanda J.
    Keeble, Anthony H.
    Davey, Martin S.
    James, Leo C.
    Eberl, Matthias
    Trowsdale, John
    JOURNAL OF IMMUNOLOGY, 2015, 194 (05): : 2390 - 2398
  • [26] Single-Turnover Activation of Arp2/3 Complex by Dip1 May Balance Nucleation of Linear versus Branched Actin Filaments
    Balzer, Connor J.
    Wagner, Andrew R.
    Helgeson, Luke A.
    Nolen, Brad J.
    CURRENT BIOLOGY, 2019, 29 (19) : 3331 - +
  • [27] Purification and characterization of an alpha(1)beta(2) isoform of CapZ from human erythrocytes: Cytosolic location and inability to bind to Mg2+ ghosts suggest that erythrocyte actin filaments are capped by adducin
    Kuhlman, PA
    Fowler, VM
    BIOCHEMISTRY, 1997, 36 (44) : 13461 - 13472
  • [28] Generation of phosphatidic acid by lysophosphatidic acid acyl transferase-β is implicated in tumorigenesis, loss of "contact inhibition," reduced actin filaments, and activation of Raf-1 kinase.
    Tang, NM
    Morrison, D
    Leung, DW
    Bonham, L
    Hollenback, DM
    Finney, RE
    CLINICAL CANCER RESEARCH, 1999, 5 : 3764S - 3764S
  • [29] Formation of smooth muscle ot actin filaments in CD34+bone marrow cells on arterial elastic laminae: Potential role of SH2 domain-containing protein tyrosine phosphatase-1
    Liu, Shu Q.
    Tefft, Brandon J.
    Zhang, Andy
    Zhang, Li-qun
    Wu, Yu H.
    MATRIX BIOLOGY, 2008, 27 (04) : 282 - 294
  • [30] CLOSE ASSOCIATION OF THE ALPHA-SUBUNITS OF G(Q) AND G(11) G-PROTEINS WITH ACTIN-FILAMENTS IN WRK(1) CELLS - RELATION TO G PROTEIN-MEDIATED PHOSPHOLIPASE-C ACTIVATION
    IBARRONDO, J
    JOUBERT, D
    DUFOUR, MN
    COHENSOLAL, A
    HOMBURGER, V
    JARD, S
    GUILLON, G
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (18) : 8413 - 8417
1234