Spectrum of cytokine gene expression in intestinal mucosal lesions of Crohn's disease and ulcerative colitis

被引：51
|
作者：
Funakoshi, K [1 ]
Sugimura, K [1 ]
Anezaki, K [1 ]
Bannai, H [1 ]
Ishizuka, K [1 ]
Asakura, H [1 ]
机构：
[1] Niigata Univ, Sch Med, Dept Internal Med 3, Niigata 951, Japan
来源：
DIGESTION | 1998年 / 59卷 / 01期
关键词：
Crohn's disease; ulcerative colitis; cytokine mRNA; reverse transcription-polymerase chain reaction;
D O I：
10.1159/000007470
中图分类号：
R57 [消化系及腹部疾病];
学科分类号：
摘要：
Background/Aims: We investigated the mRNA expression of spectrum of cytokines in the colonic mucosa in inflammatory bowel disease (IBD). Methods: The expression of cytokine gene was evaluated by using the reverse transcription-polymerase chain reaction and the radioactivity of amplified cDNA standardized by coamplified beta-actin cDNA. Results: Crohn's disease and ulcerative colitis showed significantly increased expression of IL-1 beta, IL-6, IL-8 and TNF-alpha mRNA as compared with controls (p < 0.05). Conclusion: Proinflammatory cytokines such as IL-1 beta, IL-6, IL-8 and TNF-alpha are closely involved in the immune abnormalities of inflammatory mucosal lesions in IBD.
引用
收藏
页码：73 / 78
页数：6
相关论文
共 50 条
  • [1] Mucosal inflammatory cytokine production by intestinal biopsies in patients with ulcerative colitis and Crohn's disease
    Reimund, JM
    Wittersheim, C
    Dumont, S
    Muller, CD
    Baumann, R
    Poindron, P
    Duclos, B
    [J]. JOURNAL OF CLINICAL IMMUNOLOGY, 1996, 16 (03) : 144 - 150
  • [2] Mucosal metabolism in ulcerative colitis and Crohn's disease
    Duffy, MM
    Regan, MC
    Ravichandran, P
    O'Keane, C
    Harrington, MG
    Fitzpatrick, JM
    O'Connell, PR
    [J]. DISEASES OF THE COLON & RECTUM, 1998, 41 (11) : 1399 - 1405
  • [3] Mucosal Barrier in Ulcerative Colitis and Crohn's Disease
    Dorofeyev, A. E.
    Vasilenko, I. V.
    Rassokhina, O. A.
    Kondratiuk, R. B.
    [J]. GASTROENTEROLOGY RESEARCH AND PRACTICE, 2013, 2013
  • [4] Cytokine gene expression in intestinal tuberculosis and Crohn's disease
    Pugazhendhi, S.
    Jayakanthan, K.
    Pulimood, A. B.
    Ramakrishna, B. S.
    [J]. INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE, 2013, 17 (05) : 662 - 668
  • [5] Mucosal proinflammatory cytokine and chemokine expression of gastroduodenal lesions in Crohn's disease
    Moriyama, T
    Matsumoto, T
    Jo, Y
    Yada, S
    Hirahashi, M
    Yao, T
    Iida, M
    [J]. ALIMENTARY PHARMACOLOGY & THERAPEUTICS, 2005, 21 : 85 - 91
  • [6] MUCOSAL FLORA IN CROHN'S DISEASE AND ULCERATIVE COLITIS - AN OVERVIEW
    Swidsinski, A.
    Loening-Baucke, V.
    Herber, A.
    [J]. JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 2009, 60 : 61 - 71
  • [7] Importance of mucosal healing in Crohn's disease and ulcerative colitis
    D'haens, G.
    [J]. IMMUNOREGULATION IN INFLAMMATORY BOWEL DISEASES - CURRENT UNDERSTANDING AND INNOVATION, 2007, 153 : 122 - 127
  • [8] Expression of tight junction proteins in Crohn's disease, ulcerative colitis and intestinal tuberculosis
    Das, P.
    Makharia, G. K.
    Goswami, P.
    Sreenivas, V.
    Ahuja, V.
    Gupta, S. D.
    [J]. JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY, 2010, 25 : A12 - A13
  • [9] Intestinal Neuroendocrine Tumors in Crohn's Disease and Ulcerative Colitis
    Cui, Min
    Zhang, Xiaofei
    Liao, Xiaoyan
    Liu, Qingqing
    Zhu, Hongfa
    Ko, Huaibin Mabel
    Polydorides, Alexandros D.
    Harpaz, Noam
    [J]. LABORATORY INVESTIGATION, 2018, 98 : 255 - 255
  • [10] Intestinal Neuroendocrine Tumors in Crohn's Disease and Ulcerative Colitis
    Cui, Min
    Zhang, Xiaofei
    Liao, Xiaoyan
    Liu, Qingqing
    Zhu, Hongfa
    Ko, Huaibin Mabel
    Polydorides, Alexandros D.
    Harpaz, Noam
    [J]. MODERN PATHOLOGY, 2018, 31 : 255 - 255
12345