bay scallop;
polymorphic;
simple sequence repeats;
D O I:
10.1111/j.1471-8286.2006.01606.x
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
The method of creating enriched microsatellite libraries can supply an abundant source of microsatellite sequences at a considerably reduced cost. Here we report the development of 15 polymorphic microsatellite loci from the bay scallop, Argopecten irradians, using enrichment protocol. Polymorphism was assessed in a sample of hatchery population (n = 38) revealing three to seven alleles per locus. The expected and observed heterozygosities ranged from 0.198 to 0.813 and from 0.083 to 0.833, respectively. These markers will be useful for genetic variation monitoring and parentage analysis.
机构:Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08349 USA
Song, LS
Xu, W
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机构:Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08349 USA
Xu, W
Li, CH
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机构:Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08349 USA
Li, CH
Li, HL
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机构:Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08349 USA
Li, HL
Wu, LT
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机构:Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08349 USA
Wu, LT
Xiang, JH
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机构:Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08349 USA
Xiang, JH
Guo, XM
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机构:
Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08349 USARutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08349 USA
机构:
College of Life Science and Biotechnology, Dalian Ocean University, DalianCollege of Life Science and Biotechnology, Dalian Ocean University, Dalian
Meng X.Y.
Wang X.L.
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机构:
College of Life Science and Biotechnology, Dalian Ocean University, Dalian
Key Laboratory of Mariculture and Biotechnology, Agriculture Ministry, PRC, Dalian Ocean University, DalianCollege of Life Science and Biotechnology, Dalian Ocean University, Dalian
Wang X.L.
Guo X.L.
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机构:
College of Life Science and Biotechnology, Dalian Ocean University, DalianCollege of Life Science and Biotechnology, Dalian Ocean University, Dalian
Guo X.L.
Chen M.
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机构:
Key Laboratory of Mariculture and Biotechnology, Agriculture Ministry, PRC, Dalian Ocean University, DalianCollege of Life Science and Biotechnology, Dalian Ocean University, Dalian
Chen M.
Zhang Y.M.
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机构:
College of Life Science and Biotechnology, Dalian Ocean University, DalianCollege of Life Science and Biotechnology, Dalian Ocean University, Dalian
Zhang Y.M.
Wang H.Y.
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h-index: 0
机构:
College of Life Science and Biotechnology, Dalian Ocean University, DalianCollege of Life Science and Biotechnology, Dalian Ocean University, Dalian
Wang H.Y.
Chang Y.Q.
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h-index: 0
机构:
College of Life Science and Biotechnology, Dalian Ocean University, Dalian
Key Laboratory of Mariculture and Biotechnology, Agriculture Ministry, PRC, Dalian Ocean University, DalianCollege of Life Science and Biotechnology, Dalian Ocean University, Dalian
Chang Y.Q.
Qiu X.M.
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h-index: 0
机构:
College of Life Science and Biotechnology, Dalian Ocean University, DalianCollege of Life Science and Biotechnology, Dalian Ocean University, Dalian