共 2 条
Evaluation of the Check-Points Check MDR CT103 and CT103 XL Microarray Kits by Use of Preparatory Rapid Cell Lysis
被引:26
|作者:
Cunningham, Scott A.
[1
]
Vasoo, Shawn
[1
,2
,3
]
Patel, Robin
[1
,2
]
机构:
[1] Mayo Clin, Dept Lab Med & Pathol, Div Clin Microbiol, Rochester, MN USA
[2] Mayo Clin, Dept Med, Div Infect Dis, Rochester, MN USA
[3] Tan Tock Seng Hosp, Inst Infect Dis & Epidemiol, Dept Infect Dis, Singapore, Singapore
关键词:
SPECTRUM-BETA-LACTAMASE;
GRAM-NEGATIVE BACILLI;
CARBAPENEMASE-PRODUCING ENTEROBACTERIACEAE;
REAL-TIME PCR;
ESCHERICHIA-COLI;
KLEBSIELLA-PNEUMONIAE;
MULTIPLEX PCR;
CTX-M;
DNA MICROARRAY;
UNITED-STATES;
D O I:
10.1128/JCM.03302-15
中图分类号:
Q93 [微生物学];
学科分类号:
071005 ;
100705 ;
摘要:
Using a rapid bacterial lysis method, the Check MDR CT103 and CT103 XL microarrays demonstrated accuracies of 98.1% and 94.2%, respectively, for detection of known resistance genes in 108 multidrug-resistant Gram-negative bacilli. In 45 isolates, 49 previously unrecognized extended-spectrum beta-lactamase or plasmid AmpC targets were detected and confirmed by conventional PCR.
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页码:1368 / 1371
页数:4
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