Evaluation of the Check-Points Check MDR CT103 and CT103 XL Microarray Kits by Use of Preparatory Rapid Cell Lysis

被引:26
|
作者
Cunningham, Scott A. [1 ]
Vasoo, Shawn [1 ,2 ,3 ]
Patel, Robin [1 ,2 ]
机构
[1] Mayo Clin, Dept Lab Med & Pathol, Div Clin Microbiol, Rochester, MN USA
[2] Mayo Clin, Dept Med, Div Infect Dis, Rochester, MN USA
[3] Tan Tock Seng Hosp, Inst Infect Dis & Epidemiol, Dept Infect Dis, Singapore, Singapore
关键词
SPECTRUM-BETA-LACTAMASE; GRAM-NEGATIVE BACILLI; CARBAPENEMASE-PRODUCING ENTEROBACTERIACEAE; REAL-TIME PCR; ESCHERICHIA-COLI; KLEBSIELLA-PNEUMONIAE; MULTIPLEX PCR; CTX-M; DNA MICROARRAY; UNITED-STATES;
D O I
10.1128/JCM.03302-15
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Using a rapid bacterial lysis method, the Check MDR CT103 and CT103 XL microarrays demonstrated accuracies of 98.1% and 94.2%, respectively, for detection of known resistance genes in 108 multidrug-resistant Gram-negative bacilli. In 45 isolates, 49 previously unrecognized extended-spectrum beta-lactamase or plasmid AmpC targets were detected and confirmed by conventional PCR.
引用
收藏
页码:1368 / 1371
页数:4
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