Evaluation of a DNA Microarray (Check-MDR CT102) for Rapid Detection of TEM, SHV, and CTX-M Extended-Spectrum β-Lactamases and of KPC, OXA-48, VIM, IMP, and NDM-1 Carbapenemases

被引：100

作者：

Naas, Thierry ^{[1
,2
]}

Cuzon, Gaelle ^{[1
,2
]}

Bogaerts, Pierre ^{[3
]}

Glupczynski, Youri ^{[3
]}

Nordmann, Patrice ^{[1
,2
]}

机构：

[1] Hop Bicetre, INSERM, U914, Serv Bacteriol Virol, F-94275 Le Kremlin Bicetre, France

[2] AP HP, Fac Med Paris Sud, Paris, France

[3] Clin Univ UCL Mont Godinne, Bacteriol Lab, B-5530 Yvoir, Belgium

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2011年 / 49卷 / 04期

关键词：

REAL-TIME PCR; GENES; ENTEROBACTERIACEAE; IDENTIFICATION; ASSAY; RESISTANCE; SYSTEM;

D O I：

10.1128/JCM.02607-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Check-MDR CT102 microarray, aimed at identifying bacteria producing extended-spectrum beta-lactamase (ESBL) (SHV, TEM, and CTX-M) and carbapenemase (KPC, OXA-48, VIM, IMP, and NDM-1), was evaluated on a total of 144 Gram-negative strains expressing various beta-lactamases. The sensitivity and specificity were 100% for most tested genes, suggesting that this assay allows accurate identification of common ESBL and carbapenemase producers from bacterial cultures.

引用

页码：1608 / 1613

页数：6

共 3 条

[1] Evaluation of a DNA Microarray, the Check-Points ESBL/KPC Array, for Rapid Detection of TEM, SHV, and CTX-M Extended-Spectrum β-Lactamases and KPC Carbapenemases
Naas, T.
Cuzon, G.
Truong, H.
Bernabeu, S.
Nordmann, P.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2010, 54 (08) : 3086 - 3092
[2] Evaluation of a DNA microarray for the rapid detection of extended-spectrum -lactamases (TEM, SHV and CTX-M), plasmid-mediated cephalosporinases (CMY-2-like, DHA, FOX, ACC-1, ACT/MIR and CMY-1-like/MOX) and carbapenemases (KPC, OXA-48, VIM, IMP and NDM)
Cuzon, Gaelle
Naas, Thierry
Bogaerts, Pierre
Glupczynski, Youri
Nordmann, Patrice
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2012, 67 (08) : 1865 - 1869
[3] Rapid detection of TEM, SHV and CTX-M extended-spectrum β-lactamases in Enterobacteriaceae using ligation-mediated amplification with microarray analysis
Stuart, J. Cohen
Dierikx, C.
Al Naiemi, N.
Karczmarek, A.
Van Hoek, A. H. A. M.
Vos, P.
Fluit, A. C.
Scharringa, J.
Duim, B.
Mevius, D.
Hall, M. A. Leverstein-Van
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2010, 65 (07) : 1377 - 1381

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