Analysis of underivatized gentamicin by capillary electrophoresis with UV detection

被引：36
|
作者：
Curiel, Harold [1 ]
Vanderaerden, W. [1 ]
Velez, Herman [1 ]
Hoogmartens, Jos [1 ]
Van Schepdael, Ann [1 ]
机构：
[1] Katholieke Univ Leuven, Fac Farmaceut Wetenschappen, Lab Farmaceut Analyse, B-3000 Louvain, Belgium
来源：
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2007年 / 44卷 / 01期
关键词：
gentamicin; direct UV detection; CTAB; CZE; electrosmotic flow reversal;
D O I：
10.1016/j.jpba.2007.01.029
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A selective and rapid capillary zone electrophoresis method for determination of the multicomponent aminoglycoside antibiotic gentamicin is described. Baseline separation of gentamicin C1, C1a, C2, C2a and C2b components was achieved with a background electrolyte containing 0.35 mM cetyl trimethylammonium bromide, 3% methanol and 90 mM sodium pyrophosphate (pH 7.4) and detected directly with UV detection without derivatization. Quantitative analysis was performed and illustrated the potential use of capillary electrophoresis for the identification and quantitation of gentamicin components, but the application of this method is limited to a gentamicin concentration range of 2-6 mg/ml. (c) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码：49 / 56
页数：8
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