pH-dependent aggregation and secretion of soluble monomeric influenza hemagglutinin

被引：7
|
作者：
Vanlandschoot, P [1 ]
Beirnaert, E [1 ]
Grooten, J [1 ]
Jou, WM [1 ]
Fiers, W [1 ]
机构：
[1] State Univ Ghent VIB, Mol Biol Lab, B-9000 Ghent, Belgium
来源：
ARCHIVES OF VIROLOGY | 1998年 / 143卷 / 02期
关键词：
D O I：
10.1007/s007050050282
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We previously reported the expression of soluble A/Victoria/3/75 (H3N2) hemagglutinin in insect cells and the molecular and immunological structure of an aggregated fraction, only observed in cell supernatant when expression was performed at low pH [23]. Here we report that besides this aggregated a monomeric and possibly a trimeric structure is detected in cell supernatant, irrespective of the pH of the medium. Evidence is presented that the aggregated fraction is generated out of monomeric HA0s molecules due to a low intracellular pH encountered during secretion.
引用
收藏
页码：227 / 239
页数：13
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