Comparison of emm typing and ribotyping with three restriction enzymes to characterize clinical isolates of Streptococcus pyogenes

被引：7
|
作者：
Doktor, SZ [1 ]
Beyer, JM [1 ]
Flamm, RK [1 ]
Shortridge, VD [1 ]
机构：
[1] Abbott Labs, Abbott Pk, IL 60064 USA
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2005年 / 43卷 / 01期
关键词：
D O I：
10.1128/JCM.43.1.150-155.2005
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A total of 336 Streptococcus pyogenes isolates recently recovered from patients with pharyngitis from 13 countries were characterized by emm typing and riboprinting using an automated Riboprinter (Dupont/Qualicon) based on the patterns produced by three restriction enzymes, EcoRI, PstI, and HindIII. Three enzymes were necessary to increase the discrimination of ribogroups formed by each enzyme. A total of 40 ribogroups and 38 emm sequences (not counting allelic variations) were identified. Multilocus sequence typing was performed on a sampling of the isolates, and those results were consistent with those of both emm typing and ribotyping. Correlations were observed among all three methods.
引用
收藏
页码：150 / 155
页数：6
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