Streptomyces geranii sp nov., a novel endophytic actinobacterium isolated from root of Geranium carolinianum L.

A novel endophytic actinomycete, designated A301 , was isolated from the root of Geranium carohnianurn Linn collected from Mount Emei in China and characterized using a polyphasic approach.Growth occurred at 10-37 degrees C, pH 6-11 and in the presence of 0-5 % NaCl (w/v). Strain A301(T) contained LL-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The whole-cell hydrolysates included galactose and ribose. The predominant menaquinones were MK-9(H-6) and MK-9(H-8). The major cellular fatty acids were C-15: 0' C-16: 0 anteiso-C-15: 0 and iso-C-16: 0. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, two unidentified phospholipids, three unidentified lipids and two unidentified aminophospholipids. Strain A301(T) shared the highest 16S rRNA gene sequence similarity to Streptomyces cinereoruber subsp. fructofermentans NBRC 15396(T) (98. 1 %) and Streptomyces turgidiscabies ATCC 700248(1) (98. 1 %). The DNA-DNA relatedness values between strain A301(T) and the two above-mentioned members of the genus Streptomyces were 42. 6 % and 47. 2 %, respectively. The G+C content of the DNA was 70. 5 mol%. On the basis of the polyphasic approach and DNA-DNA hybridization data, strain A301(T) represents a novel species within the genus Streptomyces, for which the name Streptomyces geranu sp. nov. is proposed. The type strain is A301(T) (=CGMCC 4. 7422(T)=JCM 32177(T)).