Isolation of adult mouse microglia using their in vitro adherent properties

被引：9

作者：

Woolf, Zoe ^{[1
,2
]}

Stevenson, Taylor J. ^{[2
,3
]}

Lee, Kevin ^{[2
,4
]}

Jung, Yewon ^{[2
,4
]}

Park, Thomas I. H. ^{[1
,2
]}

Curtis, Maurice A. ^{[2
,3
]}

Montgomery, Johanna M. ^{[2
,4
]}

Dragunow, Michael ^{[1
,2
]}

机构：

[1] Univ Auckland, Fac Med & Hlth Sci, Dept Pharmacol, Private Bag 92019, Auckland, New Zealand

[2] Univ Auckland, Ctr Brain Res, Private Bag 92019, Auckland, New Zealand

[3] Univ Auckland, Dept Anat & Med Imaging, Private Bag 92019, Auckland, New Zealand

[4] Univ Auckland, Fac Med & Hlth Sci, Dept Physiol, Private Bag 92019, Auckland, New Zealand

来源：

STAR PROTOCOLS | 2021年 / 2卷 / 02期

关键词：

Cell culture; Cell isolation; Neuroscience;

D O I：

10.1016/j.xpro.2021.100518

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Microglia are the primary innate immune effectors of the central nervous system. Although numerous protocols have been developed to isolate fetal mouse microglia, the isolation of adult mouse microglia has proven more difficult. Here, we present a simple, widely accessible protocol to isolate pure microglia cultures from 4-to 14-month-old mouse brains using their adherent properties in vitro. These isolated microglia recapitulate the adherent properties of adult human microglia and present a more suitable model for studying age-related diseases.For complete details on the use and execution of this protocol in adult human microglia, please refer to Rustenhoven et al. (2016).

引用

页数：12

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