Current status and outlook of advances in exosome isolation

被引：81

作者：

Liu, Wen-Zhao ^{[1
,2
]}

Ma, Zhan-Jun ^{[3
]}

Kang, Xue-Wen ^{[1
,2
,4
]}

机构：

[1] Lanzhou Univ, Clin Med Coll 2, Lanzhou 730030, Gansu, Peoples R China

[2] Lanzhou Univ, Dept Orthoped, Hosp 2, 82 Cuiyingmen St, Lanzhou 730030, Gansu, Peoples R China

[3] Catholic Univ Louvain, Louvain Drug Res Inst, UCLouvain, Adv Drug Delivery & Biomat, B-1200 Brussels, Belgium

[4] Int Cooperat Base Gansu Prov Pain Res Spinal Diso, Lanzhou 730000, Gansu, Peoples R China

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2022年 / 414卷 / 24期

关键词：

Exosome; Extracellular vesicle; Microfluidic; Isolation; Separation; FIELD-FLOW FRACTIONATION; EXTRACELLULAR VESICLES; WHOLE-BLOOD; SEPARATION; CAPTURE; PLASMA; SERUM; DIELECTROPHORESIS; NANOPARTICLES; BIOMARKERS;

D O I：

10.1007/s00216-022-04253-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Abstract Exosomes are extracellular vesicles with a diameter ranging from 30 to 150 nm, which are an important medium for intercellular communication and are closely related to the progression of certain diseases. Therefore, exosomes are considered promising biomarkers for the diagnosis of specific diseases, and thereby, treatments based on exosomes are being widely examined. For exosome-related research, a rapid, simple, high-purity, and recovery isolation method is the primary prerequisite for exosomal large-scale application in medical practice. Although there are no standardized methods for exosome separation and analysis, various techniques have been established to explore their biochemical and physicochemical properties. In this review, we analyzed the progress in exosomal isolation strategies and proposed our views on the development prospects of various exosomal isolation techniques.

引用

页码：7123 / 7141

页数：19

共 50 条

[21] Lactate biosensors: current status and outlook
Rassaei, Liza
Olthuis, Wouter
Tsujimura, Seiya
Sudholter, Ernst J. R.
van den Berg, Albert
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2014, 406 (01) : 123 - 137
[22] Special Issue: Resveratrol - Current Status and Outlook
Somoza, Veronika
MOLECULAR NUTRITION & FOOD RESEARCH, 2011, 55 (08) : 1127 - 1128
[23] CURRENT STATUS AND FUTURE OUTLOOK FOR TRANSPLANTATION MEDICINE
DELTZ, E
MEDIZINISCHE WELT, 1992, 43 (04): : 300 - 300
[24] The GRAPE project: Current status and future outlook
Makino, J
ASTROPHYSICAL SUPERCOMPUTING USING PARTICLE SIMULATIONS, 2003, (208): : 13 - 24
[25] Solmonella control - Current status and future outlook
Anhalt, G
FLEISCHWIRTSCHAFT, 2003, 83 (08): : 11 - 15
[26] Managing accompanying vegetation - Current status and outlook
Frochot, Henri
Armand, Gérard
Gama, Antoine
Nouveau, Marc
Wehrlen, Léon
Revue Forestiere Francaise, 2002, 54 (06): : 505 - 520
[27] Pharmacogenomics of Methotrexate: Current Status and Future Outlook
Cao, Mengda
Guo, Miao
Wu, De-Qin
Meng, Ling
CURRENT DRUG METABOLISM, 2018, 19 (14) : 1182 - 1187
[28] CONTROLLED NUCLEAR FUSION - CURRENT STATUS AND OUTLOOK
RUTSCHER, A
ENERGIETECHNIK, 1976, 26 (12): : 531 - 535
[29] Graphene in Electronics: Current Status and Future Outlook
Suemitsu, Maki
IDW'11: PROCEEDINGS OF THE 18TH INTERNATIONAL DISPLAY WORKSHOPS, VOLS 1-3, 2011, : 15 - 18
[30] Advances in exosome isolation methods and their applications in proteomic analysis of biological samples
Rui Hou
Yilan Li
Zhigang Sui
Huiming Yuan
Kaiguang Yang
Zhen Liang
Lihua Zhang
Yukui Zhang
Analytical and Bioanalytical Chemistry, 2019, 411 : 5351 - 5361

← 1 2 3 4 5 →