Simultaneous Analysis to Evaluate the Quality of Insamyangpye-Tang Using High-Performance Liquid Chromatography-Photo Diode Array Detection

Insamyangpye-tang (ISYPT) is a traditional medicinal formula comprised of 13 herbs and has been used in East Asia to treat lung-related diseases. However, to our knowledge, no method of analysis for its quality control has been reported. In this study, a method of analysis for quality control of ISYPT was developed using high-performance liquid chromatography. Chromatographic separation, analysis, and assay verification were performed with a distilled water-acetonitrile mobile phase system, both containing 0.1% (v/v) trifluoroacetic acid, and a Gemini C18 analytical column (4.6 mm x 250 mm, 5 mu m) using authentic standards for eight marker compounds. These marker constituents were detected simultaneously at 0.09-5.95 mg/g. The analysis method developed can be used for basic quality control of ISYPT.