Specificity of modified Drosophila mariner transposons in the identification of Leishmania genes

被引:3
|
作者
Augusto, MJ [1 ]
Squina, FM [1 ]
Marchini, JFM [1 ]
Dias, FC [1 ]
Tosi, LRO [1 ]
机构
[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Biol Celular & Mol & Bioagentes Patogen, BR-14049900 Ribeirao Preto, SP, Brazil
基金
巴西圣保罗研究基金会;
关键词
Leishmania; in vitro transposition; mariner transposon; gene trapping; H region;
D O I
10.1016/j.exppara.2004.08.002
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Genetic manipulation of the protozoan Leishmania has led to a better understanding of the survival and development of these pathogens within their hosts. The association of the Leishmania genome sequencing information with the ability of transposons to introduce or destroy phenotypes allows a global perspective on the role and importance of genes in cellular pathways. Herein we report the construction and testing of mariner transposable elements carrying the neomycin phosphotransferase, green fluorescent protein, or P-glucuronidase genes as reporters for translational fusion events. We demonstrate that the expression of the reporter genes will occur only when the genes are inserted in-frame within predicted genes. Our results not only add to the mariner toolkit for gene manipulation but also strengthen the evidence that the mariner system is a reliable means for the study of gene expression in Leishmania. (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:109 / 113
页数:5
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