Fabrication of a Multiple Heater-Sensor Platform for Cell Temperature Monitoring

In this paper, we present the early-stage development of a transparent device capable of maintaining the temperature of the cells of four parallel cultures. The temperature of the cells is regulated while live imaging the active area. Temperature distribution is first determined through numerical analysis and later, validated by infrared thermography. The four heater-sensor platform is fabricated using microfabrication techniques followed by an electrical characterization. The nominal set of parameters is determined. Lensfree optical imaging is performed while the system is regulating at 37 degrees C and 100 mu m diameter micro particles can be detected on the surface.