Hemin binding, functional expression, and complementation analysis of Pap31 from Bartonella henselae

被引：36
|
作者：
Zimmermann, R
Kempf, VAJ
Schiltz, E
Oberle, K
Sander, A
机构：
[1] Univ Freiburg, Inst Med Microbiol & Hyg, D-79104 Freiburg, Germany
[2] Univ Freiburg, Inst Organ Chem & Biochem, D-79104 Freiburg, Germany
[3] Univ Tubingen, Inst Microbiol, D-72074 Tubingen, Germany
来源：
JOURNAL OF BACTERIOLOGY | 2003年 / 185卷 / 05期
关键词：
D O I：
10.1128/JB.185.5.1739-1744.2003
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Growth of Bartonella henselae is strongly heme dependent, and B. henselae is unable to synthesize heme itself. At least five outer membrane-associated proteins from B. henselae bind hemin, including the 31-kDa protein designated Pap31. The gene of this protein was heterologously expressed in Escherichia coli M15(pREP4) and detected with monoclonal antibodies in the outer membrane fraction. Complementation of the hemA-deficient mutant E. coli K-12 EB53 (aroB tsx malT hemA) with pap31 demonstrated that this protein is involved in heme acquisition and may be an important virulence factor in the pathogenesis of B. henselae.
引用
收藏
页码：1739 / 1744
页数：6
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