Comparison of the protein patterns of Erysipelothrix rhusiopathiae strains by SDS-PAGE and autoradiography

被引：0
|
作者：
Bernath, S [1 ]
Kucsera, G [1 ]
Kadar, I [1 ]
Horvath, G [1 ]
Morovjan, G [1 ]
机构：
[1] Hungarian State Inst Control Vet Biol Drugs & Fee, H-1107 Budapest, Hungary
来源：
ACTA VETERINARIA HUNGARICA | 1997年 / 45卷 / 04期
关键词：
Erysipelothrix rhusiopathiae; SDS-PAGE; autoradiography; protein patterns;
D O I：
暂无
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
The proteins of 12 Erysipelothrix rhusiopathiae strains were radiolabelled with L-[S-35] methionine, and the protein fractions were detected by sodium dodecyl sulfate-polyacrylamide gel-electrophoresis (SDS-PAGE) and autoradiography. The strains were found to differ from each other in their protein patterns, thus, it was concluded that the method applied could provide useful data for the identification of strains. The described procedure was used for determining the percentage contents of protein fractions of identical molecular mass within the strains compared. The results show that there is no significant correlation between the type of the strains and the percentage of identical molecular weight fractions.
引用
收藏
页码：417 / 425
页数：9
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