Comparison of the protein patterns of Erysipelothrix rhusiopathiae strains by SDS-PAGE and autoradiography

被引：0
|
作者：
Bernath, S [1 ]
Kucsera, G [1 ]
Kadar, I [1 ]
Horvath, G [1 ]
Morovjan, G [1 ]
机构：
[1] Hungarian State Inst Control Vet Biol Drugs & Fee, H-1107 Budapest, Hungary
来源：
ACTA VETERINARIA HUNGARICA | 1997年 / 45卷 / 04期
关键词：
Erysipelothrix rhusiopathiae; SDS-PAGE; autoradiography; protein patterns;
D O I：
暂无
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
The proteins of 12 Erysipelothrix rhusiopathiae strains were radiolabelled with L-[S-35] methionine, and the protein fractions were detected by sodium dodecyl sulfate-polyacrylamide gel-electrophoresis (SDS-PAGE) and autoradiography. The strains were found to differ from each other in their protein patterns, thus, it was concluded that the method applied could provide useful data for the identification of strains. The described procedure was used for determining the percentage contents of protein fractions of identical molecular mass within the strains compared. The results show that there is no significant correlation between the type of the strains and the percentage of identical molecular weight fractions.
引用
收藏
页码：417 / 425
页数：9
相关论文
共 50 条
  • [1] SDS-PAGE protein patterns of Ascosphaera apis strains
    Chorbinski, P
    Stefaniak, T
    MEDYCYNA WETERYNARYJNA, 2004, 60 (03) : 296 - 299
  • [2] COMPARISON OF MYCOPLASMA-BOVIS STRAINS BASED ON SDS-PAGE AND IMMUNOBLOT PROTEIN-PATTERNS
    SACHSE, K
    GRAJETZKI, C
    PFUTZNER, H
    HASS, R
    JOURNAL OF VETERINARY MEDICINE SERIES B-ZENTRALBLATT FUR VETERINARMEDIZIN REIHE B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH, 1992, 39 (04): : 246 - 252
  • [3] Computerized comparison of the protein compositions of Erysipelothrix rhusiopathiae and Erysipelothrix tonsillarum strains
    Bernáth, S
    Német, L
    Tóth, K
    Morovján, G
    JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH, 2001, 48 (01): : 73 - 79
  • [4] SDS-PAGE PROTEIN-PATTERNS OF YEASTS FROM HUMAN SOURCES
    CUNNINGHAM, MJ
    NOBLE, WC
    MYCOSES, 1989, 32 (07) : 344 - 348
  • [5] Classification of cultivars of Phaseolus vulgaris according to their SDS-page protein patterns
    Stoyanova, MV
    FIRST BALKAN SYMPOSIUM ON VEGETABLES AND POTATOES, VOLS. I & II, 1997, 1 (462): : 863 - 867
  • [6] Protein and Antigenic Variability among Mycoplasma hyopneumoniae Strains by SDS-PAGE and Immunoblot
    P. Assunção
    C. De la Fe
    A. S. Ramírez
    O. González Llamazares
    J. B. Poveda
    Veterinary Research Communications, 2005, 29 : 563 - 574
  • [7] CYTOTOXICITY OF STREPTOCOCCAL EXTRACTS AND THEIR SDS-PAGE PATTERNS
    IM, MK
    LEE, SJ
    OH, SJ
    JOURNAL OF DENTAL RESEARCH, 1995, 74 : 551 - 551
  • [8] Protein and antigenic variability among Mycoplasma hyopneumoniae strains by SDS-PAGE and immunoblot
    Assunçao, P
    De la Fe, C
    Ramírez, AS
    Llamazares, OG
    Poveda, JB
    VETERINARY RESEARCH COMMUNICATIONS, 2005, 29 (07) : 563 - 574
  • [9] SDS-PAGE Heat-Shock Protein Profiles of Environmental Aeromonas Strains
    Osman, Kamelia M.
    Amin, Zeinab M. S.
    Aly, Magdy A. K.
    Hassan, Hany
    Soliman, Waleed S.
    POLISH JOURNAL OF MICROBIOLOGY, 2011, 60 (02) : 149 - 154
  • [10] PROTEIN COMPARISON OF SOYBEAN CYST NEMATODE POPULATIONS USING SDS-PAGE
    Donald, P. A.
    Patananan, A. N.
    Cerna, C. A.
    Simmons, T.
    Castro, N.
    Wilgar, J. L.
    Goheen, C.
    NEMATROPICA, 2008, 38 (02) : 137 - 144
12345