Throughout human history, gold has been the most sought-after and well-known precious metal in the world. It is synonymous with wealth in the financial market and jewellery. However, its use has a wide range of applicability in biomedicine, electronic engineering and other industries. Primary gold resources are limited and have been significantly depleted in recent decades. To cover the current demand, it is necessary to process secondary sources containing gold such as heaps, tailings, lean ores or electrical waste. New and especially more efficient technological procedures are needed for the processing of these secondary sources. From an environmental point of view, a hydrometallurgical process is considered to be more appropriate than a pyrometallurgical process, in which very toxic fumes enter the air. This work investigates the leaching of gold from flotation wastes using thiourea. It is flotation waste that is the target group of materials that are a source of precious metals. An interesting gold content is present in our waste sample (Hodru"sa Hamre tailings pond), but its evaluation depends on the setting of suitable technological conditions. In this work, thiourea is used as an alternative leaching agent to toxic cyanide, which is banned in Slovakia and many countries around the world due to its high environmental risk. The results of the study are described with respect to the gold recovery under optimized thiourea leaching conditions.