Analysis of Triclosan and Triclocarban in Soil and Biosolids Using Molecularly Imprinted Solid Phase Extraction Coupled with HPLC-UV

A molecularly imprinted polymer (MIP) able to selectively bind triclosan (TCS) and triclocarban (TCC), commonly used antibacterial agents in many consumer products, was prepared using noncovalent molecular imprinting methods. The prepared MIP was evaluated as a selective sorbent in SPE for sample cleanup before HPLC-UV analysis of TCS and TCC in soil and biosolid samples. The MIP was also compared with commercially available C-18 SPE sorbent. The molecularly imprinted SPE (MISPE) developed in this study was more efficient than C-18 SPE for the cleanup of extracts of soil and biosolid samples prior to the analysis of TCC and TCS using HPLC-UV. The LOQ values for both TCC and TCS in the soil samples were determined to be 40 mu g/kg; in the biosolid samples, the LOQ values were 100 and 300 mu g/kg for TCC and TCS, respectively. Compared to C-18 SPE, using MISPE for sample cleanup may result in a significant reduction of analytical cost, because one MIP can be reused up to 35 times and HPLC-UV instead of HPLC/MS can be used for instrumental analysis following sample cleanup by MISPE.