Synthesis of Porous CoFe2O4 and Its Application as a Peroxidase Mimetic for Colorimetric Detection of H2O2 and Organic Pollutant Degradation

Porous CoFe2O4 was prepared via a simple and controllable method to develop a low-cost, high-efficiency, and good-stability nanozyme. The morphology and microstructure of the obtained CoFe2O4 was investigated by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM), high-resolution TEM (HRTEM), specific surface area and pore analysis, and Raman spectroscopy. The results show that the annealing temperature has an important effect on the crystallinity, grain size, and specific surface area of CoFe2O4 . CoFe2O4 obtained at 300 degrees C (CF300) exhibits the largest surface area (up to 204.1 m(2) g(-1)) and the smallest grain size. The peroxidase-like activity of CoFe2O4 was further verified based on the oxidation of peroxidase substrate 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H2O2. The best peroxidase-like activity for CF300 should be ascribed to its largest surface area and smallest grain size. On this basis, an effective method of colorimetric detection H2O2 was established. In addition, the porous CoFe2O4 was also used for the catalytic oxidation of methylene blue (MB), indicating potential applications in pollutant removal and water treatment.