Ligase detection reaction and universal array as a tool to detect grapevine infecting phytoplasmas

被引：0
|
作者：
Frosini, A
Casati, P
Bianco, PA
Bordoni, R
Consolandi, C
Castiglioni, B
Mezzelani, A
Rizzi, E
Battaglia, C
Belli, G
Bernardi, LR
De Bellis, G
机构：
[1] Univ Milan, Dept Biomed Technol & Sci, LITA, I-20090 Segrate, Milan, Italy
[2] Natl Council Sci Res, Inst Agr Biotechnol & Biol, Milan, Italy
[3] Univ Milan, Inst Plant Pathol, Milan, Italy
来源：
MINERVA BIOTECNOLOGICA | 2002年 / 14卷 / 3-4期
关键词：
gene expression profiling; gene expression regulation; oligonucleotide array sequence; plant disease; Flavescence doree;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Phytoplasmas are unculturable wall less bacteria involved in many plant diseases; in particular Flavescence donee represents a severe threat to viticulture, because of its epidemic behavior in field. Here we present the application of the LDR technique, associated with hybridisation on universal arrays, as a quick and reliable tool for plant quarantine procedures and mandatory measures, in order to distinguish between different phytoplasmas' induced infections, compared to classical protocols in use, particularly PCR - RFLP assays.
引用
收藏
页码：265 / 267
页数：3
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