Investigation of a subset of human polymorphisms by ligase detection reaction and universal array

被引：0
|
作者：
Consolandi, C
Bordoni, R
Castiglioni, B
Frosini, A
Mezzelani, A
Rizzi, E
Battaglia, C
Pera, C
Ferrara, GB
Zatta, P
Zambenedetti, P
Bernardi, LR
De Bellis, G
机构：
[1] Univ Milan, Dept Biomed Sci & Technol, I-20133 Milan, Italy
[2] CNR, Inst Biomed Technol, Milan, Italy
[3] Natl Canc Inst, Inst Agr Biol & Biotechnol, Genoa, Italy
[4] Natl Canc Inst, Adv Biotechnol Ctr, Genoa, Italy
[5] Univ Genoa, Dept Oncol Biol & Genet, Genoa, Italy
[6] Gen Hosp, Encephalon Bank, Dolo Venice, Italy
来源：
MINERVA BIOTECNOLOGICA | 2002年 / 14卷 / 3-4期
关键词：
polymorphism; ligase detection reaction; universal array;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We present our results in the identification of human polymorphisms within the 2(nd) exon of the HLA-A region, in the exon 4 of apolipoprotein E and in the exon 15 of the transferrin gene. Allele specific detection was performed by PCR followed by ligase detection reaction (LDR) in combination with a universal array, a powerful method for high throughput DNA sequence analysis. This work confirmed the results previously obtained by sequence or enzymatic analysis thus demonstrating the feasibility of genotyping studies by LDR using a universal DNA array.
引用
收藏
页码：259 / 263
页数：5
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