Activation of Free Fatty Acid Receptor 4 (FFA4) Ameliorates Ovalbumin-Induced Allergic Asthma by Suppressing Activation of Dendritic and Mast Cells in Mice

被引:17
|
作者
Son, So-Eun [1 ]
Koh, Jung-Min [2 ]
Im, Dong-Soon [1 ,3 ]
机构
[1] Kyung Hee Univ, Grad Sch, Dept Biomed & Pharmaceut Sci, Seoul 02447, South Korea
[2] Univ Ulsan, Div Endocrinol & Metab, Asan Med Ctr, Coll Med, Seoul 05505, South Korea
[3] Kyung Hee Univ, Grad Sch, Dept Basic Pharmaceut Sci, Seoul 02447, South Korea
基金
新加坡国家研究基金会;
关键词
allergy; asthma; free fatty acid receptor 4; FFA4; omega-3 polyunsaturated fatty acids; DAMPENS AIRWAY INFLAMMATION; DIETARY SUPPLEMENTATION; INSULIN-RESISTANCE; RESOLVIN E1; GPR120; OMEGA-3-FATTY-ACIDS; RESOLUTION; LIPIDS; MODEL;
D O I
10.3390/ijms23095270
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Epidemiological and clinical studies have suggested that intake of n-3 polyunsaturated fatty acids (PUFA) reduces the incidence of allergic airway diseases and improves pulmonary function in patients with allergic asthma. However, the pharmacological targets of PUFA have not been elucidated upon. We investigated whether free fatty acid receptor 4 (FFA4, also known as GPR120) is a molecular target for beneficial PUFA in asthma therapy. In an ovalbumin (OVA)-induced allergic asthma model, compound A (a selective agonist of FFA4) was administrated before OVA sensitization or OVA challenge in FFA4 wild-type (WT) and knock-out (KO) mice. Compound A treatment of RBL-2H3 cells suppressed mast cell degranulation in vitro in a concentration-dependent manner. Administration of compound A suppressed in vivo allergic characteristics in bronchoalveolar lavage fluid (BALF) and lungs, such as inflammatory cytokine levels and eosinophil accumulation in BALF, inflammation and mucin secretion in the lungs. Compound A-induced suppression was not only observed in mice treated with compound A before OVA challenge, but in mice treated before OVA sensitization as well, implying that compound A acts on mast cells as well as dendritic cells. Furthermore, this suppression by compound A was only observed in FFA4-WT mice and was absent in FFA4-KO mice, implying that compound A action is mediated through FFA4. Activation of FFA4 may be a therapeutic target of PUFA in allergic asthma by suppressing the activation of dendritic cells and mast cells, suggesting that highly potent specific agonists of FFA4 could be a novel therapy for allergic asthma.
引用
收藏
页数:15
相关论文
共 50 条
  • [31] Concomitant Action of Structural Elements and Receptor Phosphorylation Determines Arrestin-3 Interaction with the Free Fatty Acid Receptor FFA4
    Butcher, Adrian J.
    Hudson, Brian D.
    Shimpukade, Bharat
    Alvarez-Curto, Elisa
    Prihandoko, Rudi
    Ulven, Trond
    Milligan, Graeme
    Tobin, Andrew B.
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2014, 289 (26) : 18451 - 18465
  • [32] Cilostazol ameliorates high free fatty acid (FFA)-induced activation of NLRP3 inflammasome in human vascular endothelial cells
    Wang, Xing
    Huang, Huiling
    Su, Chen
    Zhong, Qiaoqing
    Wu, Guifu
    ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, 2019, 47 (01) : 3704 - 3710
  • [33] Inhibition of allergic airway inflammation through the blockage of NF-κB activation by ellagic acid in an ovalbumin-induced mouse asthma model
    Zhou, Ershun
    Fu, Yunhe
    Wei, Zhengkai
    Yang, Zhengtao
    FOOD & FUNCTION, 2014, 5 (09) : 2106 - 2112
  • [34] Activation of Free Fatty Acid Receptor 4 Affects Intestinal Inflammation and Improves Colon Permeability in Mice
    Salaga, Maciej
    Bartoszek, Adrian
    Binienda, Agata
    Krajewska, Julia B.
    Fabisiak, Adam
    Mosinska, Paula
    Dziedziczak, Katarzyna
    Niewinna, Karolina
    Talar, Marcin
    Tarasiuk, Aleksandra
    Kordek, Radzislaw
    Fichna, Jakub
    NUTRIENTS, 2021, 13 (08)
  • [35] Functional expression of the free fatty acids receptor-1 and -4 (FFA1/GPR40 and FFA4/GPR120) in bovine endometrial cells
    Pamela Valenzuela
    Stefanie Teuber
    Carolina Manosalva
    Pablo Alarcón
    Carlos D. Figueroa
    Marcelo Ratto
    Rafael A. Burgos
    Maria A. Hidalgo
    Veterinary Research Communications, 2019, 43 : 179 - 186
  • [36] Bee venom ameliorates ovalbumin induced allergic asthma via modulating CD4+CD25+ regulatory T cells in mice
    Choi, Myoung Suk
    Park, Soojin
    Choi, Taewon
    Lee, Gihyun
    Haam, Kyoung-Keun
    Hong, Moo-Chang
    Min, Byung-Il
    Bae, Hyunsu
    CYTOKINE, 2013, 61 (01) : 256 - 265
  • [37] Effect of propolis phenolic compounds on free fatty acid receptor 4 activation
    Hyunnho Cho
    Kyong Kim
    Nayeon Kim
    Minji Woo
    Hye Young Kim
    Food Science and Biotechnology, 2020, 29 : 579 - 584
  • [38] Effect of propolis phenolic compounds on free fatty acid receptor 4 activation
    Cho, Hyunnho
    Kim, Kyong
    Kim, Nayeon
    Woo, Minji
    Kim, Hye Young
    FOOD SCIENCE AND BIOTECHNOLOGY, 2020, 29 (04) : 579 - 584
  • [39] Free-Fatty Acid Receptor-4 (FFA4/GPR120) differentially regulates migration, invasion, proliferation and tumor growth of papillary renal cell carcinoma cells
    Karmokar, Priyanka F.
    Moniri, Nader H.
    BIOCHEMICAL PHARMACOLOGY, 2023, 213
  • [40] Functional expression of the free fatty acids receptor-1 and-4 (FFA1/GPR40 and FFA4/GPR120) in bovine endometrial cells
    Valenzuela, Pamela
    Teuber, Stefanie
    Manosalva, Carolina
    Alarcon, Pablo
    Figueroa, Carlos D.
    Ratto, Marcelo
    Burgos, Rafael A.
    Hidalgo, Maria A.
    VETERINARY RESEARCH COMMUNICATIONS, 2019, 43 (03) : 179 - 186