Investigation of Metabolic Resistance to Soybean Aphid (Aphis glycines Matsumura) Feeding in Soybean Cultivars

Simple Summary This project examined the interaction between soybean aphids and Ontario-grown soybean cultivars to determine which leaf metabolites were most associated with aphid resistance. Tolerance and resistance were determined by measuring the growth and reproduction of aphids and leaf feeding damage over 10-day and 4-week infestation periods. Chromatographic techniques were used for the analysis of legume-specific plant natural products and isoflavonoids, and high-resolution mass spectrometry was used for the identification of free amino acids in aphid-resistant, tolerant, and susceptible soybean cultivars. There was a low correlation between isoflavonoid leaf concentrations and aphid resistance in the soybean varieties studied; however, the aphid-resistant cultivars were determined to have lower free amino acid concentrations, indicating that lower nutrient quality could be responsible for the resistance observed. Identifying these cultivars is important for managing aphid populations and provides an additional tool for soybean integrated pest management. Soybean aphid (Aphis glycines) is a major soybean (Glycine max) herbivore pest in many soybean growing regions. High numbers of aphids on soybean can cause severe reductions in yield. The management of soybean aphids includes monitoring, insecticide applications when required, and the use of resistant cultivars. Soybean aphid-resistant soybean varieties are associated with genes that confer one or more categories of resistance to soybean aphids, including antibiosis (affects survival, growth, and fecundity), antixenosis (affects behaviour such as feeding), and tolerance (plant can withstand greater damage without economic loss). The genetic resistance of soybean to several herbivores has been associated with isoflavonoid phytoalexins; however, this correlation has not been observed in soybean varieties commonly grown in southern Ontario, Canada. Isoflavonoids in the leaves of 18 cultivars in the early growth stage were analyzed by HPLC and the concentration by fresh weight was used to rate the potential resistance to aphids. Greenhouse and growth cabinet trials determined that the cultivars with greater resistance to aphids were Harosoy 63 and OAC Avatar. The most susceptible cultivar was Maple Arrow, whereas Pagoda and Conrad were more tolerant to aphid feeding damage. Overall, there was a low correlation between the number of aphids per leaf, feeding damage, and leaf isoflavonoid levels. Metabolite profiling by high-resolution LC-MS determined that the most resistant cultivar had on average lower levels of certain free amino acids (Met, Tyr, and His) relative to the most susceptible cultivar. This suggests that within the tested cultivars, nutritional quality stimulates aphid feeding more than isoflavonoids negatively affect aphid feeding or growth. These findings provide a better understanding of soybean host plant resistance and suggest ways to improve soybean resistance to aphid feeding through the breeding or metabolic engineering of leaf metabolites.