In-house phage amplification assay is a sound alternative for detecting rifampin-resistant Mycobacterium tuberculosis in low-resource settings

被引：23
|
作者：
Símboli, N
Takiff, H
McNerney, R
López, B
Martin, A
Palomino, JC
Barrera, L
Ritacco, V
机构：
[1] INEI ANLIS Carlos G Malbran, Serv Microbacterias, RA-1281 Buenos Aires, DF, Argentina
[2] INEI ANLIS Carlos G Malbran, Inst Nacl Enfermedades Infecciasas, RA-1281 Buenos Aires, DF, Argentina
[3] IVIC, Caracas, Venezuela
[4] London Sch Hyg & Trop Med, London WC1, England
[5] Inst Trop Med, Antwerp, Belgium
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2005年 / 49卷 / 01期
关键词：
D O I：
10.1128/AAC.49.1.425-427.2005
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
An in-house myrobacteriophage amplification assay for detecting rifampin-resistant Mycobacterium tuberculosis showed 100% sensitivity, 97.7% specificity, and 95.2% predictive value for resistance in a test of 129 isolates from a hot spot area of multidrug-resistant M. tuberculosis. The applicability of the test was demonstrated in the routine work flow of a low-resource reference laboratory.
引用
收藏
页码：425 / 427
页数：3
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    Liu, Guan
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    Zheng, Suhua
    Zhao, Yan-lin
    [J]. JOURNAL OF MICROBIOLOGICAL METHODS, 2013, 93 (01) : 32 - 36
  • [32] Development of an in-house loop-mediated isothermal amplification (LAMP) assay for detection of Mycobacterium tuberculosis and evaluation in sputum samples of Nepalese patients
    Pandey, Basu Dev
    Poudel, Ajay
    Yoda, Tomoko
    Tamaru, Aki
    Oda, Naozumi
    Fukushima, Yukari
    Lekhak, Binod
    Risal, Basista
    Acharya, Bishnu
    Sapkota, Bishwa
    Nakajima, Chie
    Taniguchi, Tooru
    Phetsuksiri, Benjawan
    Suzuki, Yasuhiko
    [J]. JOURNAL OF MEDICAL MICROBIOLOGY, 2008, 57 (04) : 439 - 443
  • [33] Rapid detection of drug-resistant Mycobacterium tuberculosis by Modified MODS assay suitable for resource-poor settings
    Fomda, Bashir Ahamd
    Bashir, Gulnaz
    Baqal, Sehrish
    Mir, Yaawar Bashir
    Ali, Rehana
    Khan, Altaf Hussain
    Khan, Asiya
    Bashir, Anis
    Chuloo, G. M.
    [J]. PLOS NEGLECTED TROPICAL DISEASES, 2024, 18 (01):
  • [34] Utility of nitrate reductase assay for detection of multidrug-resistant Mycobacterium tuberculosis in a low resource setting
    Lopez, Marcela
    Alvarez, Claudia
    Susana Imaz, Maria
    [J]. BIOMEDICA, 2011, 31 (02): : 258 - 263
  • [35] Discordant results between Xpert MTB/RIF assay and Bactec MGIT 960 culture system regarding the detection of rifampin-resistant Mycobacterium tuberculosis isolates in Wenzhou, China
    He, Guiqing
    Zheng, Qingyong
    Wu, Jing
    Wu, Lianpeng
    Geng, Zhi
    Jiang, Guanglu
    Huang, Hairong
    Jiang, Xiangao
    Yu, Xia
    [J]. MICROBIOLOGY SPECTRUM, 2024, 12 (06):
  • [36] Locked Nucleic Acid Probe-Based Real-Time PCR Assay for the Rapid Detection of Rifampin-Resistant Mycobacterium tuberculosis (vol 10, e0143444, 2015)
    Zhao, Yong
    Li, Guilian
    Sun, Chongyun
    Li, Chao
    Wang, Xiaochen
    Liu, Haican
    Zhang, Pingping
    Zhao, Xiuqin
    Wang, Xinrui
    Jiang, Yi
    Yang, Ruifu
    Wan, Kanglin
    Zhou, Lei
    [J]. PLOS ONE, 2016, 11 (06):
  • [37] Implementation of targeted next-generation sequencing for the diagnosis of drug-resistant tuberculosis in low-resource settings: a programmatic model, challenges, and initial outcomes
    de Araujo, Leonardo
    Cabibbe, Andrea Maurizio
    Mhuulu, Lusia
    Ruswa, Nunurai
    Dreyer, Viola
    Diergaardt, Azaria
    Gunther, Gunar
    Claassens, Mareli
    Gerlach, Christiane
    Utpatel, Christian
    Cirillo, Daniela Maria
    Nepolo, Emmanuel
    Niemann, Stefan
    [J]. FRONTIERS IN PUBLIC HEALTH, 2023, 11
  • [38] Development and evaluation of an in-house single step loop-mediated isothermal amplification (SS-LAMP) assay for the detection of Mycobacterium tuberculosis complex in sputum samples from Moroccan patients
    El Mehdi Bentaleb
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    Brahim Lakssir
    El Mostafa Ressami
    Saaïd Amzazi
    Hassan Sefrioui
    Hassan Ait Benhassou
    [J]. BMC Infectious Diseases, 16
  • [39] Development and evaluation of an in-house single step loop-mediated isothermal amplification (SS-LAMP) assay for the detection of Mycobacterium tuberculosis complex in sputum samples from Moroccan patients
    Bentaleb, El Mehdi
    Abid, Mohammed
    El Messaoudi, My Driss
    Lakssir, Brahim
    Ressami, El Mostafa
    Amzazi, Saaid
    Sefrioui, Hassan
    Benhassou, Hassan Ait
    [J]. BMC INFECTIOUS DISEASES, 2016, 16
  • [40] Implementation validation performed in Rwanda to determine whether the INNO-LiPA Rif.TB, line probe assay can be used for detection of multidrug-resistant Mycobacterium tuberculosis in low-resource countries
    Quezada, Cindy Maria
    Kamanzi, Eliane
    Mukamutara, Julienne
    De Rijk, Pim
    Rigouts, Leen
    Portaels, Francoise
    Ben Amor, Yanis
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (09) : 3111 - 3114
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