Fluorescence lifetime phantoms based on quenched coumarin derivatives

被引:0
|
作者
Freymueller, Christian [1 ]
Kalinina, Sviatlana [3 ]
Rueck, Angelika [3 ]
Sroka, Ronald [1 ,2 ]
Ruehm, Adrian [1 ,2 ]
机构
[1] Ludwig Maximilians Univ Munchen, Univ Hosp, LIFE Ctr, Laser Forschungslab, Munich, Germany
[2] Ludwig Maximilians Univ Munchen, Univ Hosp, Dept Urol, Munich, Germany
[3] Univ Ulm, Core Facil Confocal & Multiphoton Microscopy N24, Ulm, Germany
来源
ADVANCES IN MICROSCOPIC IMAGING III | 2021年 / 11922卷
关键词
STANDARDS; FAD;
D O I
10.1117/12.2615823
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Liquid reference samples for 2-photon fluorescence lifetime investigations were developed, based on coumarin 1 and 6 and 4-hydroxy-TEMPO as quencher. Spectral and fluorescence lifetime characteristics similar to intracellular NADH and FAD were achieved. (C) 2021 The Author(s)
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页数:3
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