NMR Characterization of the DNA-binding Domain of Arabidopsis thaliana Telomere Repeat Factor

被引：0
|
作者：
Lee, Inhwan [1 ]
Kim, Heeyoun [1 ]
Ko, Yoon-Joo [2 ]
Lee, Weontae [1 ]
机构：
[1] Yonsei Univ, Coll Life Sci & Biotechnol, Dept Biochem, Seoul 120749, South Korea
[2] Seoul Natl Univ, Natl Ctr Interuniv Res Facil, Seoul 151747, South Korea
来源：
BULLETIN OF THE KOREAN CHEMICAL SOCIETY | 2016年 / 37卷 / 04期
关键词：
Arabidopsis thaliana; Telomere repeat factor (AtTRF); DNA-binding motif; Nuclear magnetic resonance; REVEALS UNIQUE FEATURES; IN-VITRO; PROTEIN; SEQUENCE; TRF1; FAMILY; GENE;
D O I：
10.1002/bkcs.10708
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Telomere repeat factor (AtTRF) derived from Arabidopsis thaliana contains a myb-like domain that binds to double-stranded telomeric DNA. We cloned the myb-like domain of AtTRF (AtTRFmyb ) into a pET-15b vector and expressed the protein in Escherichia coli. AtTRFmyb was purified using Ni-affinity chromatography. DNA-binding mode has been examined by electrophoretic mobility shift assay (EMSA). Fluorescence-quenching experiments determined a K-d value of 6.62 nM between AtTRFmyb and plant telomeric DNA. Data from nuclear magnetic resonance (NMR) spectroscopy together with TALOS+ program provide the secondary structures of AtTRFmyb , suggesting that AtTRFmyb binds its plant telomere DNA with three -helices of a DNA-binding motif.
引用
收藏
页码：485 / 489
页数：5
相关论文
共 50 条
  • [1] NMR assignments and characterization of the DNA-binding domain of Arabidopsis transcription factor WRKY11
    Jiannan Wang
    Yaling Lin
    Qinjun Zhu
    Jingfeng Zhang
    Maili Liu
    Yunfei Hu
    [J]. Magnetic Resonance Letters, 2021, 1 (02) : 112 - 120
  • [2] NMR assignments and characterization of the DNA-binding domain of Arabidopsis transcription factor WRKY11
    Wang, Jiannan
    Lin, Yaling
    Zhu, Qinjun
    Zhang, Jingfeng
    Liu, Maili
    Hu, Yunfei
    [J]. MAGNETIC RESONANCE LETTERS, 2021, 1 (02) : 112 - 120
  • [3] NMR Study of Consensus DNA-binding Site for Arabidopsis thaliana Class I Transcription Factor AtTCP1
    Choi, Yong-Geun
    Kim, Hee-Eun
    Lee, Joon-Hwa
    [J]. JOURNAL OF THE KOREAN MAGNETIC RESONANCE SOCIETY, 2013, 17 (02): : 76 - 80
  • [4] Arabidopsis thaliana telomeric DNA-binding protein 1 is required for telomere length homeostasis and its Myb-extension domain stabilizes plant telomeric DNA binding
    Hwang, Moo Gak
    Cho, Myeon Haeng
    [J]. NUCLEIC ACIDS RESEARCH, 2007, 35 (04) : 1333 - 1342
  • [5] Characterization of a mitochondrially targeted single-stranded DNA-binding protein in Arabidopsis thaliana
    Edmondson, AC
    Song, DQ
    Alvarez, LA
    Wall, MK
    Almond, D
    McClellan, DA
    Maxwell, A
    Nielsen, BL
    [J]. MOLECULAR GENETICS AND GENOMICS, 2005, 273 (02) : 115 - 122
  • [6] Characterization of a mitochondrially targeted single-stranded DNA-binding protein in Arabidopsis thaliana
    Andrew C. Edmondson
    Daqing Song
    Luis A. Alvarez
    Melisa K. Wall
    David Almond
    David A. McClellan
    Anthony Maxwell
    Brent L. Nielsen
    [J]. Molecular Genetics and Genomics, 2005, 273 : 115 - 122
  • [7] Structural approach to a novel tandem repeat DNA-Binding domain, STPR, by CD and NMR
    Saito, Shin
    Aizawa, Tomoyasu
    Kawaguchi, Kyosuke
    Yamaki, Takeshi
    Matsumoto, Daisuke
    Kamiya, Masakatsu
    Kumaki, Yasuhiro
    Mizuguchi, Mineyuki
    Takiya, Sigeharu
    Demura, Makoto
    Kawano, Keiichi
    [J]. BIOCHEMISTRY, 2007, 46 (07) : 1703 - 1713
  • [8] NMR Solution Structure and DNA-binding Model of the DNA-binding Domain of Competence Protein A
    Hobbs, Carey A.
    Bobay, Benjamin G.
    Thompson, Richele J.
    Perego, Marta
    Cavanagh, John
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 2010, 398 (02) : 248 - 263
  • [9] ISOLATION OF A DNA-BINDING DOMAIN FROM THE EUPLOTES TELOMERE PROTEIN
    PRICE, CM
    SKOPP, R
    [J]. MOLECULAR BIOLOGY OF THE CELL, 1992, 3 : A136 - A136
  • [10] Identification of a consensus DNA-binding site for the Arabidopsis thaliana SBP domain transcription factor, AtSPL14, and binding kinetics by surface plasmon resonance
    Liang, Xinwen
    Nazarenus, Tara J.
    Stone, Julie M.
    [J]. BIOCHEMISTRY, 2008, 47 (12) : 3645 - 3653
12345