NMR Study of Consensus DNA-binding Site for Arabidopsis thaliana Class I Transcription Factor AtTCP1

被引:4
|
作者
Choi, Yong-Geun [1 ]
Kim, Hee-Eun [1 ]
Lee, Joon-Hwa [1 ]
机构
[1] Gyeongsang Natl Univ, RINS, Jinju 660701, Gyeongnam, South Korea
来源
基金
新加坡国家研究基金会;
关键词
NMR; DNA binding; Hydrogen exchange; TCP transcription factor;
D O I
10.6564/JKMRS.2013.17.2.076
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The TCP domain is a DNA-binding domain present in plant transcription factors and has a similar structural feature to the bHTH motif of eukaryotic transcription factors. The imino proton exchange study has been performed for the DNA duplex containing the consensus DNA-binding site for the AtTCP11 transcription factor. The first two base pairs in the consensus 5'-GTGGG-3' sequence are relatively very unstable but lead to greater stabilization of the neighboring two G center dot C base pairs. These unique dynamic features of the five base pairs in the consensus DNA sequence might play crucial roles in the effective DNA binding of the AtTCP11 protein.
引用
收藏
页码:76 / 80
页数:5
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