Cloning and molecular characterization of BmHYA1, a novel hyaluronidase from the venom of Chinese red scorpion Buthus martensi Karsch

被引：7
|
作者：
Feng, Luo [1 ]
Gao, Rong [1 ]
Meng, Jun
Gopalakrishnakone, Ponnampalam [1 ]
机构：
[1] Natl Univ Singapore, Venom & Toxin Res Programme, Dept Anat, Yong Loo Lin Sch Med, Singapore 117597, Singapore
来源：
TOXICON | 2010年 / 56卷 / 03期
关键词：
Scorpion venom hyaluronidase; Full protein sequence; 3 ' RACE PCR; Edman degradation; Phylogenetic tree; ANTIGENIC CROSS-REACTIVITY; BEE VENOM; SEQUENCE; ALLERGEN; HORNET;
D O I：
10.1016/j.toxicon.2010.04.009
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
In this communication, the full protein sequence of a novel venom hyaluronidase BmHYA1 was reported. It is the first full hyaluronidase amino acid sequence from scorpion venom. It was deduced from nucleotide sequence by 3' Rapid Amplification of cDNA Ends (RACE) PCR cloning, followed by alignment with the N-terminal amino acid sequence, which was obtained by Edman degradation. BmHYA1 has 385 amino acid residues containing five potential N-glycosylation sites. The phylogenetic analysis indicates early divergence and independent evolution of BmHYA1 from other hyaluronidases. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码：474 / 479
页数：6
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