Genetic alterations in a telomerase-immortalized human esophageal epithelial cell line: Implications for carcinogenesis

被引：25
|
作者：
Cheung, Pak Yan [1 ]
Deng, Wen [1 ]
Man, Cornelia [1 ]
Tse, Wan Wai [1 ]
Srivastava, Gopesh [2 ]
Law, Simon [3 ]
Tsao, Sai Wah [1 ]
Cheung, Annie L. M. [1 ]
机构：
[1] Univ Hong Kong, Li Ka Shing Fac Med, Dept Anat, Canc Biol Grp, Pokfulam, Hong Kong, Peoples R China
[2] Univ Hong Kong, Li Ka Shing Fac Med, Dept Pathol, Pokfulam, Hong Kong, Peoples R China
[3] Univ Hong Kong, Li Ka Shing Fac Med, Dept Surg, Pokfulam, Hong Kong, Peoples R China
来源：
CANCER LETTERS | 2010年 / 293卷 / 01期
关键词：
Esophageal; Telomerase; Immortalization; p16(INK4a); Karyotype; BARRETTS-ESOPHAGUS; REVERSE-TRANSCRIPTASE; PROMOTER REGION; T-ANTIGEN; KERATINOCYTES; ABERRATIONS; P16(INK4A); EXPRESSION; DYSPLASIA; GROWTH;
D O I：
10.1016/j.canlet.2009.12.015
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Ectopic expression of viral oncoproteins disrupts cellular functions and limits the value of many existing immortalization models as models for carcinogenesis, especially for cancers without definitive viral etiology. Our newly established telomerase-immortalized human esophageal epithelial cell line, NE2-hTERT, retained nearly-diploid and non-tumorigenic characteristics, but exhibited genetic and genomic alterations commonly found in esophageal cancer, including progressive loss of the p16(INK4a) alleles, upregulation of anti-apoptotic proteins, epithelial-mesenchymal transition, whole-chromosome 7 gain and duplicated 5q arm. Our data also revealed a novel positive regulation of p16(INK4a) on cyclin D1. These findings probably represent early crucial events and mechanisms in esophageal carcinogenesis. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：41 / 51
页数：11
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