Combined use of the fully automated Bactec MGIT 960 System and a PCR-restriction fragment length polymorphism analysis for routine detection and identification of mycobacteria from clinical samples

被引：8
|
作者：
Kontos, F
Petinaki, E
Gitti, Z
Costopoulos, C
Anagnostou, S
Tselentis, I
Maniatis, AN
机构：
[1] Univ Hosp Larissa, Dept Microbiol, Sch Med, Larisa, Greece
[2] Univ Hosp Crete, Dept Clin Bacteriol, Iraklion, Greece
[3] Gen Peripheral Hosp Chest Dis Sotiria, Natl Mycobacterium Reference Unit, Athens, Greece
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2003年 / 52卷 / 01期
关键词：
Bactec MGIT 960 system; identification; PCR-RFLP analysis; mycobacteria;
D O I：
10.1016/S0167-7012(02)00137-9
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The combined use of Bactec MGIT 960 system and a PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis was assessed for the rapid detection and identification of mycobacteria from clinical samples. The diagnostic sensitivity and the time of detection of MGIT 960 system were significantly higher than of Lowenstein -Jensen medium. PCR-RFLP identification analysis results were in concordance with those obtained by the conventional biochemical tests. (C) 2003 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：137 / 140
页数：4
相关论文
共 50 条
  • [21] Use of PCR-restriction fragment length polymorphism analysis to identify the main new world Leishmania species and analyze their taxonomic properties and polymorphism by application of the assay to clinical samples
    Rotureau, B
    Ravel, C
    Couppié, P
    Pratlong, F
    Nacher, M
    Dedet, JP
    Carme, B
    JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (02) : 459 - 467
  • [22] Identification of Bartonella species directly in clinical specimens by PCR-restriction fragment length polymorphism analysis of a 16S rRNA gene fragment
    Matar, GM
    Koehler, JE
    Malcolm, G
    Lambert-Fair, MA
    Tappero, J
    Hunter, SB
    Swaminathan, B
    JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (12) : 4045 - 4047
  • [23] Development of a species-specific PCR-restriction fragment length polymorphism analysis procedure for identification of Madurella mycetomatis
    Ahmed, AOA
    Mukhtar, MM
    Kools-Sijmons, M
    Fahal, AH
    de Hoog, S
    Van den Ende, BG
    Zijlstra, EE
    Verbrugh, H
    Abugroun, ESAM
    Elhassan, AM
    van Belkum, A
    JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (10) : 3175 - 3178
  • [24] Rapid identification of Borrelia burgdorferi sensu lato by PCR-restriction fragment length polymorphism analysis of groEL gene
    Park, HS
    Lee, JH
    Jeong, EJ
    Park, TK
    Jang, WJ
    Park, KH
    Kim, BJ
    Kook, YH
    Lee, SH
    MICROBIOLOGY AND IMMUNOLOGY, 2004, 48 (11) : 893 - 897
  • [25] PCR-restriction fragment length polymorphism analysis for identification of Bacteroides spp. and characterization of nitroimidazole resistance genes
    Stubbs, SLJ
    Brazier, JS
    Talbot, PR
    Duerden, BI
    JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (09) : 3209 - 3213
  • [26] Identification of 54 mycobacterial species by PCR-restriction fragment length polymorphism analysis of the hsp65 gene
    Brunello, F
    Ligozzi, M
    Cristelli, E
    Bonora, S
    Tortoli, E
    Fontana, R
    JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (08) : 2799 - 2806
  • [27] Use of PCR-restriction fragment length polymorphism for the identification of zoonotic mycobacteriosis in zebrafish caused by Mycobacterium abscessus and Mycobacterium chelonae
    Seok, Seung-Hyeok
    Koo, Hye Cheong
    Kasuga, Asako
    Kim, Yeun
    Lee, Eun Gae
    Lee, Hyeyoung
    Park, Jong-Hwan
    Baek, Min-Won
    Lee, Hui-Young
    Kim, Dong-Jae
    Lee, Byeung-Hee
    Lee, Yong-Soon
    Cho, Sang-Nae
    Park, Jae-Hak
    VETERINARY MICROBIOLOGY, 2006, 114 (3-4) : 292 - 297
  • [28] flaB Gene as a Molecular Marker for Distinct Identification of Borrelia Species in Environmental Samples by the PCR-Restriction Fragment Length Polymorphism Method
    Wodecka, Beata
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2011, 77 (19) : 7088 - 7092
  • [29] Evaluation of PCR-restriction profile analysis and IS2404 restriction fragment length polymorphism and amplified fragment length polymorphism fingerprinting for identification and typing of Mycobacterium ulcerans and M-marinum
    Chemlal, K
    Huys, G
    Fonteyne, PA
    Vincent, V
    Lopez, AG
    Rigouts, L
    Swings, J
    Meyers, WM
    Portaels, F
    JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (09) : 3272 - 3278
  • [30] Identification of oral Peptostreptococcus isolates by PCR-restriction fragment length polymorphism analysis of 16S rRNA genes
    Riggio, MP
    Lennon, A
    JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (09) : 4475 - 4479
12345