Combined use of the fully automated Bactec MGIT 960 System and a PCR-restriction fragment length polymorphism analysis for routine detection and identification of mycobacteria from clinical samples

被引：8
|
作者：
Kontos, F
Petinaki, E
Gitti, Z
Costopoulos, C
Anagnostou, S
Tselentis, I
Maniatis, AN
机构：
[1] Univ Hosp Larissa, Dept Microbiol, Sch Med, Larisa, Greece
[2] Univ Hosp Crete, Dept Clin Bacteriol, Iraklion, Greece
[3] Gen Peripheral Hosp Chest Dis Sotiria, Natl Mycobacterium Reference Unit, Athens, Greece
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2003年 / 52卷 / 01期
关键词：
Bactec MGIT 960 system; identification; PCR-RFLP analysis; mycobacteria;
D O I：
10.1016/S0167-7012(02)00137-9
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The combined use of Bactec MGIT 960 system and a PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis was assessed for the rapid detection and identification of mycobacteria from clinical samples. The diagnostic sensitivity and the time of detection of MGIT 960 system were significantly higher than of Lowenstein -Jensen medium. PCR-RFLP identification analysis results were in concordance with those obtained by the conventional biochemical tests. (C) 2003 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：137 / 140
页数：4
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