Kinetic mechanism for the formation of the presynaptic complex of the bacterial recombinase RecA

被引:7
|
作者
Defais, M [1 ]
Phez, E [1 ]
Johnson, NP [1 ]
机构
[1] CNRS, UMR 5089, Inst Pharmacol & Biol Struct, F-31077 Toulouse, France
关键词
D O I
10.1074/jbc.M204341200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
RecA protein from Escherichia coli catalyzes DNA strand exchange during homologous recombination in a reaction that requires nucleoside triphosphate cofactor. In the first step of this reaction RecA protein polymerizes on single-stranded DNA to form a filament with a stoichiometry of three nucleotides/RecA monomer called the presynaptic complex. We have used fluorescence anisotropy of a fluorescein-labeled oligonucleotide to investigate presynaptic complex formation. RecA-ATPgammaS bound to oligonucleotide by a two-step process. Kinetic studies revealed an intermediate in the polymerization reaction that had greater mobility, than the final product filament. The intermediate was transformed into the final product by a process that was independent of filament concentration and temperature, k = 0.3 +/- 0.1 min(-1). This process had the same rate as that reported for a step in the isomerization of presynaptic complex by ATPgammaS (Paulus, B. F., and Bryant, F. R. (1997) Biochemistry 36, 7832-7838). Judging from anisotropy measurements, the intermediate had hydrodynamic properties similar to a mixed filament containing RecA monomers with and without ATPgammaS. These results show that the presynaptic complex can assume conformations with different segmental mobilities that could play a role in homologous recombination.
引用
收藏
页码:3545 / 3551
页数:7
相关论文
共 50 条
  • [31] Linear dichroism reveals the perpendicular orientation of DNA bases in the RecA and Rad51 recombinase filaments: A possible mechanism for the strand exchange reaction
    Takahashi, Masayuki
    Ito, Kentaro
    Iwasaki, Hiroshi
    Norden, Bengt
    CHIRALITY, 2024, 36 (04)
  • [32] MECHANISM OF COMPLEX FORMATION IN SULFATE SOLUTIONS
    GORSKI, N
    JOURNAL OF INORGANIC & NUCLEAR CHEMISTRY, 1969, 31 (02): : 275 - &
  • [33] Donor–Acceptor Mechanism of Complex Formation
    S. S. Batsanov
    Russian Journal of Coordination Chemistry, 2002, 28 : 1 - 5
  • [34] MECHANISM OF DIKE FORMATION OF COMPLEX STRUCTURE
    DENICENK.VK
    DOKLADY AKADEMII NAUK SSSR, 1965, 162 (03): : 664 - &
  • [35] FORMATION OF A THIAMINE DISULFIDE COMPLEX WITH FATTY-ACID - MECHANISM OF FORMATION OF THE COMPLEX
    KOMATA, Y
    FUJIE, T
    KANEKO, A
    UEDA, F
    URANO, S
    LIPIDS, 1988, 23 (06) : 525 - 527
  • [36] On kinetic and macroscopic models for the stripe formation in engineered bacterial populations
    Jiang, Ning
    Liang, Jiangyan
    Luo, Yi-Long
    Tang, Min
    Zhang, Yaming
    JOURNAL OF DIFFERENTIAL EQUATIONS, 2022, 323 : 38 - 85
  • [37] Kinetic identification of a mechanism of complex plasma chemical reactions
    Zyn, VI
    PLASMA CHEMISTRY AND PLASMA PROCESSING, 1998, 18 (03) : 395 - 408
  • [38] Kinetic Identification of a Mechanism of Complex Plasma Chemical Reactions
    V. I. Zyn
    Plasma Chemistry and Plasma Processing, 1998, 18 : 395 - 408
  • [39] Kinetic mechanism of nitric oxide-inhibited mitochondrial complex III. Effect on ubisemiquinone and superoxide formation
    Iglesias, Dario E.
    Bombicino, Silvina S.
    Boveris, Alberto
    Valdez, Laura B.
    FREE RADICAL BIOLOGY AND MEDICINE, 2019, 139 : S29 - S29
  • [40] A kinetic approach to the mechanism of formation of mesoporous silica nanoparticles
    Meza, Ricardo Alvarado
    Santori, Tom
    Cattoen, Xavier
    JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY, 2023, 114 (1) : 56 - 64