The [Mo6Cl14]2- Cluster is Biologically Secure and Has Anti-Rotavirus Activity In Vitro

被引：6

作者：

Rojas-Mancilla, Edgardo ^{[1
]}

Oyarce, Alexis ^{[2
]}

Verdugo, Viviana ^{[2
]}

Morales-Verdejo, Cesar ^{[3
]}

Echeverria, Cesar ^{[3
]}

Velasquez, Felipe ^{[4
]}

Chnaiderman, Jonas ^{[4
]}

Valiente-Echeverria, Fernando ^{[4
]}

Ramirez-Tagle, Rodrigo ^{[5
]}

机构：

[1] Univ Bernardo O Higgins, Dept Ciencias Quim & Biol, Gen Gana 1702, Santiago 8370854, Chile

[2] Univ Bernardo O Higgins, Escuela Tecnol Med, Gen Gana 1702, Santiago 8370854, Chile

[3] Univ Bernardo O Higgins, Ctr Integrat Biol & Quim Aplicada, Gen Gana 1702, Santiago 8370854, Chile

[4] Univ Chile, Programa Virol, Inst Ciencias Biomed, Independencia 1027, Independencia 8380453, Chile

[5] Univ Bernardo O Higgins, Fac Ingn Ciencia & Tecnol, Ave Viel 1497, Santiago 8370993, Chile

来源：

MOLECULES | 2017年 / 22卷 / 07期

关键词：

cluster; cell viability; hemolysis; red blood cells; albumin; rotavirus; MOLYBDENUM OCTAHEDRAL CLUSTERS; HUMAN SERUM-ALBUMIN; SILICA NANOPARTICLES; ELECTRONIC-STRUCTURE; MOLECULAR-PROPERTIES; CELLULAR UPTAKE; HEPATOMA-CELLS; COMPLEXES; LUMINESCENCE; CYTOTOXICITY;

D O I：

10.3390/molecules22071108

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The molybdenum cluster [Mo6Cl14](2-) is a fluorescent component with potential for use in cell labelling and pharmacology. Biological safety and antiviral properties of the cluster are as yet unknown. Here, we show the effect of acute exposition of human cells and red blood cells to the molybdenum cluster and its interaction with proteins and antiviral activity in vitro. We measured cell viability of HepG2 and EA.hy926 cell lines exposed to increasing concentrations of the cluster (0.1 to 250 mu M), by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay. Hemolysis and morphological alterations of red blood cells, obtained from healthy donors, exposed to the cluster (10 to 200 mu M) at 37 degrees C were analyzed. Furthermore, quenching of tryptophan residues of albumin was performed. Finally, plaque formation by rotavirus SA11 in MA104 cells treated with the cluster (100 to 300 mu M) were analyzed. We found that all doses of the cluster showed similar cell viability, hemolysis, and morphology values, compared to control. Quenching of tryptophan residues of albumin suggests a protein-cluster complex formation. Finally, the cluster showed antiviral activity at 300 mu M. These results indicate that the cluster [Mo6Cl14](2-) could be intravenously administered in animals at therapeutic doses for further in vivo studies and might be studied as an antiviral agent.

引用

页数：12

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