Preparation and study on the solid inclusion complex of sparfloxacin with HP-β-cyclodextrin

The interaction of sparfloxacin with HP-beta-cyclodextrin (HP-beta-CD) has been studied by several analytical techniques, including H-1 NMR, fluorescence spectroscopy, infrared spectroscopy, thermal analysis and scanning electron microscopy. In this paper, solid inclusion complex of sparfloxacin with HP-beta-CD was synthesized by the coprecipitation method. In addition, the characterization of the inclusion complex has been proved by fluorimetry, infrared, differential scanning calorimetry and ID, 2D NMR. The experimental results confirmed the existence of 1:1 inclusion complex of sparfloxacin with HP-beta-CD. The formation constant of complex was determined by the fluorescence method and H-1 NMR. Spacial configuration of complex has been proposed on 2D NMR technique. (C) 2002 Elsevier Science B.V. All rights reserved.